Distinct transcriptional modules in the peripheral blood mononuclear cells response to human respiratory syncytial virus or to human rhinovirus in hospitalized infants with bronchiolitis

Autor(es)

Vieira, Sandra E. ^[1] ; Bando, Silvia Y. ^[1] ; de Paulis, Milena ^[2] ; Oliveira, Danielle B. L. ^[3] ; Thomazelli, Luciano M. ^[3] ; Durigon, Edison L. ^[3] ; Martinez, Marina B. ^[2] ; Moreira-Filho, Carlos Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pediat, Fac Med, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Univ Hosp, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 14, n. 3 MAR 7 2019.
• Citações Web of Science: 0

Resumo

Human respiratory syncytial virus (HRSV) is the main cause of bronchiolitis during the first year of life, when infections by other viruses, such as rhinovirus, also occur and are clinically indistinguishable from those caused by HRSV. In hospitalized infants with bronchiolitis, the analysis of gene expression profiles from peripheral blood mononuclear cells (PBMC) may be useful for the rapid identification of etiological factors, as well as for developing diagnostic tests, and elucidating pathogenic mechanisms triggered by different viral agents. In this study we conducted a comparative global gene expression analysis of PBMC obtained from two groups of infants with acute viral bronchiolitis who were infected by HRSV (HRSV group) or by HRV (HRV group). We employed a weighted gene co-expression network analysis (WGCNA) which allows the identification of transcriptional modules and their correlations with HRSV or HRV groups. This approach permitted the identification of distinct transcription modules for the HRSV and HRV groups. According to these data, the immune response to HRSV infection comparatively to HRV infection was more associated to the activation of the interferon gamma signaling pathways and less related to neutrophil activation mechanisms. Moreover, we also identified host-response molecular markers that could be used for etiopathogenic diagnosis. These results may contribute to the development of new tests for respiratory virus identification. The finding that distinct transcriptional profiles are associated to specific host responses to HRSV or to HRV may also contribute to the elucidation of the pathogenic mechanisms triggered by different respiratory viruses, paving the way for new therapeutic strategies. (AU)

• Processo FAPESP: 15/22308-2 - Representações intermediárias em Ciência Computacional para descoberta de conhecimento
• Beneficiário: Roberto Marcondes Cesar Junior
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/22854-9 - Análise do perfil de expressão gênica em células mononucleares do sangue periférico de lactentes com infecção por vírus sincicial respiratório
• Beneficiário: Sandra Elisabete Vieira
• Linha de fomento: Auxílio à Pesquisa - Regular