Dual-species biofilms of Streptococcus mutans and Candida albicans exhibit more biomass and are mutually beneficial compared with single-species biofilms

Autor(es)

Vitorino Lobo, Carmelia Isabel ^[1] ; Rinaldi, Talita Baptista ^[1] ; Somogyi Christiano, Chiara Mikaella ^[1] ; Leite, Luana De Sales ^[1] ; Barbugli, Paula Aboud ^[1] ; Klein, Marlise Inez ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Sao Paulo, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL MICROBIOLOGY; v. 11, n. 1 JAN 1 2019.
• Citações Web of Science: 0

Resumo

Background: Streptococcus mutans (Sm) and Candida albicans (Ca) are found in biofilms of early childhood caries. Objective: To characterize in vitro dual- and single-species biofilms of Sm and Ca formed on saliva-coated hydroxyapatite discs in the presence of sucrose. Design: Evaluation of biofilms included biochemical {[}biomass, proteins, matrix's water-soluble (WSP) and alkali-soluble (ASP) polysaccharides, microbiological, 3D structure, gene expression, and stress tolerance analyses. Results: Biomass and proteins were higher for dual-species and lower for Ca (p = 0.001). Comparison of Sm single- and dual-species biofilms revealed no significant difference in Sm numbers or quantity of WSP (p > 0.05). Dual-species biofilms contained a higher population of Ca (p < 0.001). The quantity of ASP was higher in dual-species biofilms (vs Ca single-species biofilms; p = 0.002). The 3D structure showed larger microcolonies and distinct distribution of Sm-derived exopolysaccharides in dual-species biofilms. Compared with dual-species biofilms, expression of gtfB (ASP) and nox1 (oxidative stress) was higher for single-species of Sm whilst expression of BGL2 (matrix), PHR1 (matrix, acid tolerance) and SOD1 (oxidative stress) was higher in single-species of Ca. There was no difference for acid tolerance genes (Sm atpD and Ca PHR2), which was confirmed by acid tolerance challenge. Dual-species biofilms were more tolerant to oxidative and antimicrobial stresses (p < 0.05). Conclusions: Dual-species biofilms present greater 3D complexity, thereby, making them more resistant to stress conditions. (AU)

• Processo FAPESP: 16/10833-8 - Patogenicidade da interação de Streptococcus mutans e Candida albicans em biofilme oral
• Beneficiário: Talita Baptista Rinaldi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/08021-5 - Análise da expressão de genes de Streptococcus mutans em biofilme misto
• Beneficiário: Luana de Sales Leite
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico