Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dual-species biofilms of Streptococcus mutans and Candida albicans exhibit more biomass and are mutually beneficial compared with single-species biofilms

Full text
Author(s):
Vitorino Lobo, Carmelia Isabel [1] ; Rinaldi, Talita Baptista [1] ; Somogyi Christiano, Chiara Mikaella [1] ; Leite, Luana De Sales [1] ; Barbugli, Paula Aboud [1] ; Klein, Marlise Inez [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Sao Paulo, Araraquara - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF ORAL MICROBIOLOGY; v. 11, n. 1 JAN 1 2019.
Web of Science Citations: 0
Abstract

Background: Streptococcus mutans (Sm) and Candida albicans (Ca) are found in biofilms of early childhood caries. Objective: To characterize in vitro dual- and single-species biofilms of Sm and Ca formed on saliva-coated hydroxyapatite discs in the presence of sucrose. Design: Evaluation of biofilms included biochemical {[}biomass, proteins, matrix's water-soluble (WSP) and alkali-soluble (ASP) polysaccharides, microbiological, 3D structure, gene expression, and stress tolerance analyses. Results: Biomass and proteins were higher for dual-species and lower for Ca (p = 0.001). Comparison of Sm single- and dual-species biofilms revealed no significant difference in Sm numbers or quantity of WSP (p > 0.05). Dual-species biofilms contained a higher population of Ca (p < 0.001). The quantity of ASP was higher in dual-species biofilms (vs Ca single-species biofilms; p = 0.002). The 3D structure showed larger microcolonies and distinct distribution of Sm-derived exopolysaccharides in dual-species biofilms. Compared with dual-species biofilms, expression of gtfB (ASP) and nox1 (oxidative stress) was higher for single-species of Sm whilst expression of BGL2 (matrix), PHR1 (matrix, acid tolerance) and SOD1 (oxidative stress) was higher in single-species of Ca. There was no difference for acid tolerance genes (Sm atpD and Ca PHR2), which was confirmed by acid tolerance challenge. Dual-species biofilms were more tolerant to oxidative and antimicrobial stresses (p < 0.05). Conclusions: Dual-species biofilms present greater 3D complexity, thereby, making them more resistant to stress conditions. (AU)

FAPESP's process: 16/10833-8 - Pathogenicity of Streptococcus mutans and Candida albicans interaction in oral biofilm
Grantee:Talita Baptista Rinaldi
Support Opportunities: Scholarships in Brazil - Scientific Initiation