Annexin A1 peptide and endothelial cell-conditioned medium modulate cervical tumorigenesis

Autor(es)

Cardin, Laila Toniol ^[1] ; Prates, Janesly ^[1] ; da Cunha, Bianca Rodrigues ^[2] ; Tajara, Eloiza Helena ^[2] ; Oliani, Sonia Maria ^[1] ; Rodrigues-Lisoni, Flavia Cristina ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Humanities & Exact Sci, Sao Jose Do Rio Preto - Brazil

^[2] Sch Med Sao Jose Do Rio Preto, Dept Mol Biol, Sao Jose Do Rio Preto - Brazil

^[3] Sao Paulo State Univ Unesp, Sch Engn, Ilha Solteira - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS OPEN BIO; v. 9, n. 4, p. 668-681, APR 2019.
• Citações Web of Science: 0

Resumo

Cervical cancer is one of the leading causes of cancer death in women worldwide, and its tumorigenesis can be influenced by the microenvironment. The anti-inflammatory protein annexin A1 (ANXA1) has been reported to be associated with cancer progression and metastasis, suggesting that it plays a role in regulating tumour cell proliferation. Here, we examined the effect of the N-terminal peptide Ac2-26 of ANXA1 on the HaCaT cell line (normal) and HeLa cell line (cervical cancer) co-cultured with endothelium cell-conditioned medium (HMC). Treatment with Ac2-26 decreased proliferation and increased motility of cervical cancer cells, but did not affect cellular morphology or viability. Combined HMC stimulus and Ac2-26 treatment resulted in an increase in apoptotic HeLa cells, upregulated expression of MMP2, and downregulated expression of COX2, EP3 and EP4. In conclusion, Ac2-26 treatment may modulate cellular and molecular mechanisms underlying cervical carcinogenesis. (AU)

• Processo FAPESP: 16/02012-4 - Investigação das atividades imunomoduladoras da proteína anexina A1 na regulação inflamatória de doenças do sistema gastrointestinal: estudo em modelos experimentais in vivo e in vitro
• Beneficiário: Sonia Maria Oliani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/24083-2 - Investigação da modulação da proteína anti-inflamatória anexina A1 em diferentes células de câncer de colo de útero
• Beneficiário: Flávia Cristina Rodrigues Lisoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular