Should we ban total phenolics and antioxidant screening methods? The link between antioxidant potential and activation of NF-kappa B using phenolic compounds from grape by-products

Autor(es)

de Camargo, Adriano Costa ^{[1, 2]} ; Telles Biasoto, Aline Camarao ^[3] ; Schwember, Andres R. ^[1] ; Granato, Daniel ^[4] ; Rasera, Gabriela Boscariol ^[2] ; Franchin, Marcelo ^[5] ; Rosalen, Pedro L. ^[5] ; Alencar, Severino Matias ^[2] ; Shahidi, Fereidoon ^[6]

Afiliação do(s) autor(es)

^[1] Pontificia Univ Catolica Chile, Fac Agron & Ingn Forestal, Dept Ciencias Vegetales, Casilla 306-22, Santiago - Chile

^[2] Univ Sao Paulo, Dept Agrifood Ind Food & Nutr, Luiz de Queiroz Coll Agr, Av Padua Dias 11, POB 9, BR-13418900 Piracicaba, SP - Brazil

^[3] Embrapa Semiarido, Rodovia BR 428, Km 152, POB 23, BR-56302970 Petrolina, PE - Brazil

^[4] Univ Estadual Ponta Grossa, Dept Food Engn, Av Carlos Cavalcanti 4748, BR-84030900 Ponta Grossa - Brazil

^[5] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP - Brazil

^[6] Mem Univ Newfoundland, Dept Biochem, St John, NF A1B 3X9 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 290, p. 229-238, AUG 30 2019.
• Citações Web of Science: 7

Resumo

Free radical imbalance is associated with several chronic diseases. However, recent controversies have put in check the validity of colorimetric methods to screen the functionality of polyphenols. Therefore, in this study two antioxidant methods, based on chemical reactions, were tested for their ability in anticipating the reduction of the activation of NF-kappa 3 using LPS-activated RAW 264.7 macrophages, selected as a biological model. Grape processing by-products from winemaking showed higher total phenolic content (TPC), antioxidant capacity towards peroxyl radical (31.1%) as well as reducing power (39.5%) than those of grape juice by-products. The same trend was observed when these samples were tested against LPS-activated RAW 264.7 macrophages by reducing the activation NF-kappa B. Feedstocks containing higher TPC and corresponding ORAC and FRAP results translated to higher reduction in the activation of NF-kappa 3 (36.5%). Therefore, this contribution demonstrates that colorimetric methods are still important screening tools owing their simplicity and widespread application. (AU)

• Processo FAPESP: 08/55492-7 - Prospeccao e identificacao de compostos bioativos de residuos agroindustriais para aplicacao em alimentos e bebidas.
• Beneficiário: Severino Matias de Alencar
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/15563-9 - Caracterização de novos alvos farmacológicos e estudo da atividade de uma 4-fenil cumarina na diferenciação de linfócitos Th17 no desenvolvimento da doença periodontal
• Beneficiário: Marcelo Franchin
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado