Biodegradation of anthracene and different PAHs by a yellow laccase from Leucoagaricus gongylophorus

Autor(es)

Leme Ike, Priscila Tomie ^{[1, 2]} ; Birolli, Willian Garcia ^[3] ; dos Santos, Danilo Martins ^[3] ; Meleiro Porto, Andre Luiz ^[3] ; Ferreira Souza, Dulce Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, Dept Quim, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Inst Fed Parana, Rua Antonio Carlos Rodrigues 453, BR-87703539 Paranagua, PR - Brazil

^[3] Univ Sao Paulo, Lab Quim Organ & Biocatalise, Inst Quim Sao Carlos, Av Joao Dagnone 1100, Ed Quim Ambiental, BR-13563120 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 26, n. 9, p. 8675-8684, MAR 2019.
• Citações Web of Science: 0

Resumo

Laccases produced by Leucoagaricus gongylophorus act in lignocellulose degradation and detoxification processes. Therefore, the use of L. gongylophorus laccase (Lac1Lg) was proposed in this work for degradation of anthracene and others polycyclic aromatic hydrocarbons without the use of mediators. Degradation reactions were performed in buffer aqueous solution with 10 ppm of anthracene and other PAHs, Tween-20 in 0.25% v/v and a laccase preparation of 50 U. The optimum condition (pH 6.0 and 30 degrees C) was determined by response surface methodology with an excellent coefficient of determination (R-2) of 0.97 and an adjusted coefficient of determination (R-adj(2)) of 0.93. In addition, the employment of the mediator ABTS decreased the anthracene biodegradation from 44 +/- 1% to 30 +/- 1%. This optimum pH of 6.0 suggests that the reaction occurs by a hydrogen atom transfer mechanism. Additionally, in 24 h Lac1Lg biodegraded 72 +/- 1% anthracene, 40 +/- 3% fluorene and 25 +/- 3% phenanthrene. The yellow laccase from L. gongylophorus biodegraded anthracene and produced anthrone and anthraquinone, which are interesting compounds for industrial applications. Moreover, this enzyme also biodegraded the PAHs phenanthrene and fluorene justifying the study of Lac1Lg for bioremediation of these compounds in the environment. (AU)

• Processo FAPESP: 12/19934-0 - Biodegradação de pesticidas por micro-organismos (fungos e bactérias)
• Beneficiário: Andre Luiz Meleiro Porto
• Modalidade de apoio: Auxílio à Pesquisa - Regular