Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients

Autor(es)

Silvana Aparecida Alves Corrêa de Noronha ^[1] ; Samuel Marcos Ribeiro de Noronha ^[2] ; Larissa Elias Lanziani ^[3] ; Michele Zampieri Ipolito ^[4] ; Lydia Masako Ferreira ^[5] ; Alfredo Gragnani ^[6]

Afiliação do(s) autor(es)

^[1] UNIFESP. EPM. Department of Surgery - Brasil

^[2] UNIFESP. EPM. Department of Surgery - Brasil

^[3] UNIFESP. EPM - Brasil

^[4] UNIFESP. EPM - Brasil

^[5] UNIFESP. EPM. Department of Surgery - Brasil

^[6] Federal University of Sao Paulo. Paulista School of Medicine. Department of Surgery - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, p. 39-43, 2014-00-00.

Resumo

PURPOSE: To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples. METHODS: Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR. RESULTS: In fibroblasts, KGF gene expression was 220±80 and 33.33±6.67 (M±SD; N=4), respectively for major and minor burn groups. In keratinocytes, KGF gene expression was 11.2±1.9 and 3.45±0.37 (M±SD; N=4), respectively for major and minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0±4.0 and 11.5±0.5 (M±SD; N=4), respectively for major and minor burn. In keratinocyte, HBD-4 gene expression was 0.0±0.0 and 13.4±4.8 (M±SD; N=4), respectively for major and minor burn. CONCLUSIONS: KGF expression was increased in burn patient fibroblasts compared to control group. In keratinocytes culture, KGF suppression is inversely proportional to burn extension; it is active and increased in major burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts and decreased in keratinocytes from all burned patients. (AU)

• Processo FAPESP: 11/12945-4 - Fator de crescimento de queratinócitos, interleucinas 1 beta, 6, 8, 10, 12 e fator de necrose tumoral alfa em pacientes com queimadura
• Beneficiário: Alfredo Gragnani Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular