Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients

Author(s)

Silvana Aparecida Alves Corrêa de Noronha ; Samuel Marcos Ribeiro de Noronha ; Larissa Elias Lanziani ; Michele Zampieri Ipolito ; Lydia Masako Ferreira ; Alfredo Gragnani

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 29, p. -, 2014.

Abstract

PURPOSE: To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples. METHODS: Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR. RESULTS: In fibroblasts, KGF gene expression was 220±80 and 33.33±6.67 (M±SD; N=4), respectively for major and minor burn groups. In keratinocytes, KGF gene expression was 11.2±1.9 and 3.45±0.37 (M±SD; N=4), respectively for major and minor burn groups. In fibroblasts, HBD-4 gene expression was 15.0±4.0 and 11.5±0.5 (M±SD; N=4), respectively for major and minor burn. In keratinocyte, HBD-4 gene expression was 0.0±0.0 and 13.4±4.8 (M±SD; N=4), respectively for major and minor burn. CONCLUSIONS: KGF expression was increased in burn patient fibroblasts compared to control group. In keratinocytes culture, KGF suppression is inversely proportional to burn extension; it is active and increased in major burn but decreased in minor burn. HBD-4 expression was increased in fibroblasts and decreased in keratinocytes from all burned patients. (AU)

• FAPESP's process: 11/12945-4 - Keratinocyte growth factor, interleukin 1 beta, 6, 8, 10, 12 and tumor necrosis factor alpha in burned patients
• Grantee: Alfredo Gragnani Filho
• Support type: Regular Research Grants