Variable sources of Bk virus in renal allograft recipients

Autor(es)

Urbano, Paulo Roberto P. ^[1] ; da Silva Nali, Luiz H. ^[1] ; Oliveira, Renato dos R. ^[1] ; Sumita, Laura M. ^[1] ; da Silva Fink, Maria Cristina D. ^[1] ; Pierrotti, Ligia C. ^{[2, 3]} ; Bicalho, Camila da Silva ^[2] ; David-Neto, Elias ^{[2, 3]} ; Pannuti, Claudio S. ^[1] ; Romano, Camila M. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Med Trop Sao Paulo, Virol Lab, Rua Dr Eneas de Carvalho Aguiar 470, BR-05403000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Hosp Clin, Div Molestias Infecciosas & Parasitarias, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Hosp Clin, Serv Transplante Renal, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP LIM52, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 91, n. 6, p. 1136-1141, JUN 2019.
• Citações Web of Science: 0

Resumo

BK virus is the causative agent of polyomavirus-associated nephropathy, a major cause of kidney transplant failure affecting 1%-10% of recipients. Previous studies that investigated the viral source on the kidney recipient pointed that the donor is implicated in the origin of human polyomavirus BK (BKPyV) infection in recipients, but giving the low genetic variability of BKPyV this subject is still controversial. The aim of this study was to determine if BKPyV replicating in kidney recipients after transplantation is always originated from the donor. Urine and blood samples from 68 pairs of living donors and kidney recipients who underwent renal transplantation from August 2010-September 2011 were screened for BKPyV by real time polymerase chain reaction. Only three recipients presented viremia. When both donors and recipients were BKPyV positive, a larger fragment of VP1 region was obtained and sequenced to determine the level of similarity between them. A phylogenetic tree was built for the 12 pairs of sequences obtained from urine and high level of similarity among all sequences was observed, indicating that homology inferences for donor and recipient viruses must be cautiously interpreted. However, a close inspection on the donor-recipient pairs sequences revealed that 3 of 12 pairs presented considerably different viruses and 4 of 12 presented mixed infection, indicating that the source of BKPyV infection is not exclusively derived from the donor. We report that about 60% of the renal recipients shed BKPyV genetically distinct from the donor, confronting the accepted concept that the donor is the main source of recipients' infection. (AU)

• Processo FAPESP: 10/15114-3 - Determinação da frequência de reativação e caracterização molecular dos poliomavírus BK e JC em receptores de transplante renal
• Beneficiário: Claudio Sergio Pannuti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/01710-3 - Determinação da prevalência e caracterização molecular dos poliomavírus humanos - bk e jc - em pacientes submetidos a transplante renal
• Beneficiário: Renato dos Reis Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado