Previously undescribed thyroid-specific miRNA sequences in papillary thyroid carcinoma

Autor(es):Mostrar menos -

Barros-Filho, Mateus Camargo ^{[1, 2]} ; Pewarchu, Michelle ^[2] ; Minatel, Brenda De Carvalho ^[2] ; Sage, Adam P. ^[2] ; Marshall, Erin A. ^[2] ; Martinez, Victor D. ^[2] ; Rock, Leigha D. ^{[2, 3, 4]} ; MacAulay, Gavin ^[2] ; Kowalski, Luiz Paulo ^[1] ; Rogatto, Silvia Regina ^[5] ; Garnis, Cathie ^[2] ; Lam, Wan L. ^[2]

Afiliação do(s) autor(es)

^[1] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo - Brazil

^[2] British Columbia Canc Res Ctr, Dept Integrat Oncol, Vancouver, BC - Canada

^[3] British Columbia Canc Res Ctr, Dept Canc Control Res, Vancouver, BC - Canada

^[4] Univ British Columbia, Fac Dent, Vancouver, BC - Canada

^[5] Univ Southern Denmark, Vejle Hosp, Inst Reg Hlth Res, Odense - Denmark

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF HUMAN GENETICS; v. 64, n. 5, p. 505-508, MAY 2019.
• Citações Web of Science: 2

Resumo

Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, wherein diagnostic limitations and lack of accurate prognostic factors are important clinical challenges. In this study, we report the discovery of 234 novel miRNAs in non-neoplastic thyroid and PTC samples, obtained from publicly available small RNA sequencing datasets (TCGA and GEO). These sequences were observed to display similar molecular features compared to currently annotated miRNAs. These potentially novel miRNAs presented tissue-specificity and largely decreased expression in PTC compared to nonneoplastic samples. We showed that the disrupted novel miRNAs have diagnostic and prognostic potential, and were associated with BRAF mutation, a frequent alteration related to more aggressive PTC. In conclusion, our results expand the miRNA repertoire in thyroid tissues and highlight the potential biological role and clinical utility of previously unannotated miRNAs. (AU)

• Processo FAPESP: 18/06138-8 - Pequenos RNAs não codificadores como reguladores chave no câncer de tireóide
• Beneficiário: Mateus de Camargo Barros Filho
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/17707-5 - Desenvolvimento de métodos moleculares úteis para o diagnóstico diferencial de nódulos da tireóide
• Beneficiário: Mateus de Camargo Barros Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/20748-5 - Marcadores diagnósticos e prognósticos aplicáveis na prática clínica para pacientes com nódulos tireoidianos: validação de achados prévios e estudos funcionais
• Beneficiário: Silvia Regina Rogatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular