Potential of oxygen and nitrogen reactive intermediates to disperse Listeria monocytogenes from biofilms

Autor(es)

dos Reis-Teixeira, Fernanda Barbosa ^[1] ; Conceicao, Natalia ^[1] ; da Silva, Lilian Pereira ^[1] ; Alves, Virginia Farias ^[2] ; Pereira De Martinis, Elaine Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCFRP, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Goias, Fac Farm, Rua 240, Esquina Com 5a Ave, S-N Setor Leste Univ, BR-74605170 Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 50, n. 2, p. 501-506, APR 2019.
• Citações Web of Science: 0

Resumo

Studying biofilm dispersal is important to prevent Listeria monocytogenes persistence in food processing plants and to avoid finished product contamination. Reactive oxygen and nitrogen intermediates (ROI and RNI, respectively) may trigger cell detachment from many bacterial species biofilms, but their roles in L. monocytogenes biofilms have not been fully investigated. This study reports on ROI and RNI quantification in Listeria monocytogenes biofilms formed on stainless steel and glass surfaces; bacterial culture and microscopy combined with fluorescent staining were employed. Nitric oxide (NO) donor and inhibitor putative effects on L. monocytogenes dispersal from biofilms were evaluated, and transcription of genes (prfA, lmo 0990, lmo 0807, and lmo1485) involved in ROI and RNI stress responses were quantified by real-time PCR (qPCR). Microscopy detected the reactive intermediates NO, peroxynitrite, H2O2, and superoxide in L. monocytogenes biofilms. Neither NO donor nor inhibitors interfered in L. monocytogenes growth and gene expression, except for lmo0990, which was downregulated. In conclusion, ROI and RNI did not exert dispersive effects on L. monocytogenes biofilms, indicating that this pathogen has a tight control for protection against oxidative and nitrosative stresses. (AU)

• Processo FAPESP: 04/08868-0 - Consolidação do Centro de Microscopia Funcional do Campus da USP/ Ribeirão Preto
• Beneficiário: Roy Edward Larson
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 11/07062-6 - Estudo da formação e dispersão de biofilmes por Listeria monocytogenes em superfície abiótica
• Beneficiário: Elaine Cristina Pereira de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/10051-3 - Avaliação do papel de óxido nítrico, de óleos essenciais e de sanitizantes na dispersão de biofilmes de Listeria monocytogenes em superfície abiótica
• Beneficiário: Fernanda Barbosa dos Reis Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado