Potential of oxygen and nitrogen reactive intermediates to disperse Listeria monocytogenes from biofilms

Author(s)

dos Reis-Teixeira, Fernanda Barbosa ^[1] ; Conceicao, Natalia ^[1] ; da Silva, Lilian Pereira ^[1] ; Alves, Virginia Farias ^[2] ; Pereira De Martinis, Elaine Cristina ^[1]

Affiliation

^[1] Univ Sao Paulo, FCFRP, Av Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Goias, Fac Farm, Rua 240, Esquina Com 5a Ave, S-N Setor Leste Univ, BR-74605170 Goiania, Go - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 50, n. 2, p. 501-506, APR 2019.
• Web of Science Citations: 0

Abstract

Studying biofilm dispersal is important to prevent Listeria monocytogenes persistence in food processing plants and to avoid finished product contamination. Reactive oxygen and nitrogen intermediates (ROI and RNI, respectively) may trigger cell detachment from many bacterial species biofilms, but their roles in L. monocytogenes biofilms have not been fully investigated. This study reports on ROI and RNI quantification in Listeria monocytogenes biofilms formed on stainless steel and glass surfaces; bacterial culture and microscopy combined with fluorescent staining were employed. Nitric oxide (NO) donor and inhibitor putative effects on L. monocytogenes dispersal from biofilms were evaluated, and transcription of genes (prfA, lmo 0990, lmo 0807, and lmo1485) involved in ROI and RNI stress responses were quantified by real-time PCR (qPCR). Microscopy detected the reactive intermediates NO, peroxynitrite, H2O2, and superoxide in L. monocytogenes biofilms. Neither NO donor nor inhibitors interfered in L. monocytogenes growth and gene expression, except for lmo0990, which was downregulated. In conclusion, ROI and RNI did not exert dispersive effects on L. monocytogenes biofilms, indicating that this pathogen has a tight control for protection against oxidative and nitrosative stresses. (AU)

• FAPESP's process: 11/07062-6 - Study of formation and dispersal of Listeria monocytogenes biofilms on abiotic surface
• Grantee: Elaine Cristina Pereira de Martinis
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/10051-3 - Evaluation the role of nitric oxide, essential oils and sanitizers in the dispersion of biofilms of Listeria monocytogenes on abiotic surface
• Grantee: Fernanda Barbosa dos Reis Teixeira
• Support Opportunities: Scholarships in Brazil - Doctorate