| Texto completo | |
| Autor(es): |
Silva, Larissa G.
[1]
;
Lorenzetti, Alan P. R.
[2]
;
Ribeiro, Rodolfo A.
[1]
;
Alves, Ingrid R.
[1]
;
Leaden, Laura
[1]
;
Galhardo, Rodrigo S.
[1]
;
Koide, Tie
[2]
;
Marques, Marilis V.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, Ribeirao Preto - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Gene; v. 700, p. 70-84, JUN 5 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Oxidative stress generated by hydrogen peroxide is faced by bacteria when encountering hostile environments. In order to define the physiological and regulatory networks controlling the oxidative stress response in the freeliving bacterium Caulobacter crescentus, a whole transcriptome analysis of wild type and AoxyR strains in the presence of hydrogen peroxide for two different exposure times was carried out. The C. crescentus response to H2O2 includes a decrease of the assimilative sulfate reduction and a shift in the amino acid synthesis pathways into favoring the synthesis of histidine. Moreover, the expression of genes encoding enzymes for the depolymerization of polyhydroxybutyrate was increased, and the RpoH-dependent genes were severely repressed. Based on the expression pattern and sequence analysis, we postulate that OxyR is probably directly required for the induction of three genes (katG, ahpCF). The putative binding of OxyR to the ahpC regulatory region could be responsible for the use of one of two alternative promoters in response to oxidative stress. Nevertheless, OxyR is required for the expression of 103 genes in response to H2O2. Fur and part of its regulon were differentially expressed in response to hydrogen peroxide independently of OxyR. The non-coding RNA OsrA was upregulated in both strains, and an in silica analysis indicated that it may have a regulatory role. This work characterizes the physiological response to H2O2 in C. crescentus, the regulatory networks and differentially regulated genes in oxidative stress and the participation of OxyR in this process. It is proposed that besides OxyR, a second layer of regulation may be achieved by a small regulatory RNA and other transcriptional regulators. (AU) | |
| Processo FAPESP: | 15/07386-7 - Identificação de RNAs regulatórios em resposta à disponibilidade de ferro e seus mecanismos de ação em Caulobacter crescentus |
| Beneficiário: | Laura Leaden |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/06378-3 - Caracterização da DEAD box-RNA helicase CC1478 em Caulobacter crescentus e sua regulação |
| Beneficiário: | Rodolfo Alvarenga Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/21038-1 - O transcritoma antisense da archaea halofílica Halobacterium salinarum |
| Beneficiário: | Tie Koide |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/04046-8 - Sistemas regulatórios da resposta bacteriana a estresses |
| Beneficiário: | Marilis Do Valle Marques |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/03052-2 - RNAs não codificantes derivados de sequências de inserção na arqueia halófila Halobacterium salinarum NRC-1 |
| Beneficiário: | Alan Péricles Rodrigues Lorenzetti |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |