Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

OxyR and the hydrogen peroxide stress response in Caulobacter crescentus

Texto completo
Autor(es):
Silva, Larissa G. [1] ; Lorenzetti, Alan P. R. [2] ; Ribeiro, Rodolfo A. [1] ; Alves, Ingrid R. [1] ; Leaden, Laura [1] ; Galhardo, Rodrigo S. [1] ; Koide, Tie [2] ; Marques, Marilis V. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Bioquim & Imunol, Fac Med Ribeirao Preto, Ribeirao Preto - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Gene; v. 700, p. 70-84, JUN 5 2019.
Citações Web of Science: 0
Resumo

Oxidative stress generated by hydrogen peroxide is faced by bacteria when encountering hostile environments. In order to define the physiological and regulatory networks controlling the oxidative stress response in the freeliving bacterium Caulobacter crescentus, a whole transcriptome analysis of wild type and AoxyR strains in the presence of hydrogen peroxide for two different exposure times was carried out. The C. crescentus response to H2O2 includes a decrease of the assimilative sulfate reduction and a shift in the amino acid synthesis pathways into favoring the synthesis of histidine. Moreover, the expression of genes encoding enzymes for the depolymerization of polyhydroxybutyrate was increased, and the RpoH-dependent genes were severely repressed. Based on the expression pattern and sequence analysis, we postulate that OxyR is probably directly required for the induction of three genes (katG, ahpCF). The putative binding of OxyR to the ahpC regulatory region could be responsible for the use of one of two alternative promoters in response to oxidative stress. Nevertheless, OxyR is required for the expression of 103 genes in response to H2O2. Fur and part of its regulon were differentially expressed in response to hydrogen peroxide independently of OxyR. The non-coding RNA OsrA was upregulated in both strains, and an in silica analysis indicated that it may have a regulatory role. This work characterizes the physiological response to H2O2 in C. crescentus, the regulatory networks and differentially regulated genes in oxidative stress and the participation of OxyR in this process. It is proposed that besides OxyR, a second layer of regulation may be achieved by a small regulatory RNA and other transcriptional regulators. (AU)

Processo FAPESP: 15/07386-7 - Identificação de RNAs regulatórios em resposta à disponibilidade de ferro e seus mecanismos de ação em Caulobacter crescentus
Beneficiário:Laura Leaden
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 16/06378-3 - Caracterização da dead box-RNA helicase CC1478 em Caulobacter crescentus e sua regulação
Beneficiário:Rodolfo Alvarenga Ribeiro
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 15/21038-1 - O transcritoma antisense da Archaea halofílica Halobacterium salinarum
Beneficiário:Tie Koide
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/04046-8 - Sistemas regulatórios da resposta bacteriana a estresses
Beneficiário:Marilis Do Valle Marques
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 17/03052-2 - RNAs não codificantes derivados de sequências de inserção na arqueia halófila Halobacterium salinarum NRC-1
Beneficiário:Alan Péricles Rodrigues Lorenzetti
Linha de fomento: Bolsas no Brasil - Doutorado