Heparin is biocompatible and can induce differentiation of human dental pulp cells

Autor(es)

Rodrigues, E. M. ^[1] ; Cornelio, A. L. G. ^[1] ; Godoi, P. H. ^[2] ; da Costa, I, P. ; Rossa-Junior, C. ^[3] ; Faria, G. ^[1] ; Guerreiro Tanomaru, J. M. ^[1] ; Tanomaru-Filho, M. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Dept Restorat Dent, Dent Sch, UNESP, Araraquara, SP - Brazil

^[2] Natl Inst Metrol Qual & Technol, Rio De Janeiro - Brazil

^[3] Sao Paulo State Univ, Dept Diag & Surg, UNESP, Dent Sch, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 6, p. 829-837, JUN 2019.
• Citações Web of Science: 0

Resumo

Aim To investigate the biocompatibility, osteogenic bioactivity and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). Methodology hDPCs were exposed to the heparin, and cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay, and the detection of calcium deposits by alizarin red staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t-test (alpha = 0.05). Results Heparin had no cytotoxic effect and did not induce apoptosis. After 3 days, heparin had significantly higher ALP activity in comparison with the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expressions were significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Conclusions Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs. (AU)

• Processo FAPESP: 11/13116-1 - Avaliação da citotoxicidade, genotoxicidade e bioatividade de diferentes tipos de cimento Portland e associações radiopacificadores micro e nanoparticulados
• Beneficiário: Ana Lívia Gomes Cornélio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/15278-6 - Avaliação dos efeitos da terapia fotodinâmica com hipericina no tratamento da doença periodontal induzida em ratos
• Beneficiário: Rosemary Adriana Chierici Marcantonio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03437-6 - Avaliação do preparo reciprocante, da obturação dos canais radiculares, e das propriedades físico-químicas e biológicas de materiais obturadores e reparadores
• Beneficiário: Mario Tanomaru Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular