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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Heparin is biocompatible and can induce differentiation of human dental pulp cells

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Author(s):
Rodrigues, E. M. [1] ; Cornelio, A. L. G. [1] ; Godoi, P. H. [2] ; da Costa, I, P. ; Rossa-Junior, C. [3] ; Faria, G. [1] ; Guerreiro Tanomaru, J. M. [1] ; Tanomaru-Filho, M. [1]
Total Authors: 8
Affiliation:
[1] Sao Paulo State Univ, Dept Restorat Dent, Dent Sch, UNESP, Araraquara, SP - Brazil
[2] Natl Inst Metrol Qual & Technol, Rio De Janeiro - Brazil
[3] Sao Paulo State Univ, Dept Diag & Surg, UNESP, Dent Sch, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Endodontic Journal; v. 52, n. 6, p. 829-837, JUN 2019.
Web of Science Citations: 0
Abstract

Aim To investigate the biocompatibility, osteogenic bioactivity and mRNA expression of the osteo/odontogenic markers bone morphogenetic protein 2 (BMP-2), osteocalcin (OC) and alkaline phosphatase (ALP), induced by heparin in human dental pulp cells (hDPCs). Methodology hDPCs were exposed to the heparin, and cell viability was assessed by 3-(4,5-dimethylthiazol)-2,5-diphenyltetrazolium bromide (MTT), and cell death was evaluated by flow cytometry. Osteogenic bioactivity was evaluated by the alkaline phosphatase (ALP) assay, and the detection of calcium deposits by alizarin red staining (ARS). The gene expression of BMP-2, OC and ALP was quantified with real-time PCR. Statistical analysis was performed with ANOVA and Bonferroni or Tukey post-test and t-test (alpha = 0.05). Results Heparin had no cytotoxic effect and did not induce apoptosis. After 3 days, heparin had significantly higher ALP activity in comparison with the control (P < 0.05). Heparin had a significant (P < 0.05) stimulatory effect on the formation of mineralized nodules. BMP-2 and OC mRNA expressions were significantly higher in cells exposed to heparin than control group after 1 day (P < 0.05). Conclusions Heparin was biocompatible in hDPCs, induced osteogenic bioactivity and enhanced mRNA expression of osteo/odontogenic markers BMP-2 and OC. These results suggest that heparin has potential to induce osteo/odontogenic cell differentiation of hDPCs. (AU)

FAPESP's process: 11/13116-1 - Cytotoxicity, genotoxicity and bioactivity of different types of Portland cement and associations with micro and nanoparticles radiopacifiers
Grantee:Ana Lívia Gomes Cornélio
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/15278-6 - Photodynamic therapy with hypericin on the treatment of periodontal disease induced in rats
Grantee:Rosemary Adriana Chierici Marcantonio
Support Opportunities: Regular Research Grants
FAPESP's process: 15/03437-6 - Evaluation of reciprocating preparation, root canal filling, and the physicochemical and biological properties of filling and repair materials
Grantee:Mario Tanomaru Filho
Support Opportunities: Regular Research Grants