Development and analysis of a bilayer scaffold for pulp-dentin regeneration

• Grant number: 17/22739-9
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2018
• End date: November 30, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Carlos Alberto de Souza Costa
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Diana Gabriela Soares dos Passos ; Giovana Anovazzi Medeiros ; Josimeri Hebling Costa

Abstract

The development of biocompatible and bioactive scaffolds based on biotechnology and tissue engineering concepts is the goal of pulp-dentin complex regeneration. These biomaterials should be able to induce the chemotaxis of resident stem cells to the pulp exposure site, followed by odontoblastic differentiation and deposition of mineralized extracellular matrix. For this specific clinical situation, the biomaterial surface in direct contact with the pulp tissue should modulate dentin regeneration mediated by stem cells, while the opposite face should be able to promote pulpal surface seal. In the present study, the development of a bilayer chitosan scaffold consisting of a dense surface interposed to a bioactive macro-porous surface will be proposed, aiming the development of innovative biomaterial for direct pulp capping therapy. Initially, a dense layer capable of co-polymerization with liner materials and avoid the diffusion of toxic components through its structure will be developed. Then, a bioactive scaffold, containing an interconnected porous network will be prepared, and finally the bi-layer scaffold will be stablished by the interposition of both biomaterials. The potential of bi-layer scaffold to act as a pulp capping agent will be evaluated by means of an artificial pulp chamber with simulated pulp pressure in vitro model (pAPC). The capability of dental pulp cells to migrate from a 3D culture to the scaffold surface, as well as its odontoblastic differentiation, will be evaluated. The numerical data obtained by the application of laboratory protocols will be submitted to specific statistical analysis. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORTECHO-ZUTA, UXUA; DE OLIVEIRA DUQUE, CARLA CAROLINE; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; FRAGA BRISO, ANDRE LUIZ; SOUZA COSTA, CARLOS ALBERTO. Polymeric biomaterials maintained the esthetic efficacy and reduced the cytotoxicity of in-office dental bleaching. Journal of Esthetic and Restorative Dentistry, v. 33, n. 8, JUL 2021. (17/22739-9)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; ANSELMI, CAROLINE; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Signaling Biomaterial for Dental Pulp Regeneration. JOURNAL OF ENDODONTICS, v. 47, n. 7, p. 1110-1117, JUL 2021. (17/14210-8, 17/22739-9)

RIBEIRO, R. A. O.; DE OLIVEIRA DUQUE, C. C.; ORTECHO-ZUTA, U.; LEITE, M. L.; HEBLING, J.; SOARES, D. G.; DE SOUZA COSTA, C. A.. Influence of Manganese Oxide on the Esthetic Efficacy and Toxicity Caused by Conventional In-office Tooth Bleaching Therapy. Operative Dentistry, v. 47, n. 4, p. 12-pg., 2022-07-01. (17/22739-9)

KITAGAWA, FERNANDA ALI; LEITE, MARIA LUISA; MENDES SOARES, IGOR PAULINO; ANSELMI, CAROLINE; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Influence of ceramic veneer on the transdentinal cytotoxicity, degree of conversion and bond strength of light-cured resin cements to dentin. Dental Materials, v. 38, n. 6, p. 14-pg., 2022-05-26. (17/22739-9)

ORTECHO-ZUTA, UXUA; DE OLIVEIRA DUQUE, CARLA CAROLINE; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; BRISO, ANDRE LUIZ FRAGA; DE SOUZA COSTA, CARLOS ALBERTO. Strategy for reducing cytotoxicity and obtaining esthetic efficacy with 15 min of in-office dental bleaching. CLINICAL ORAL INVESTIGATIONS, v. 26, n. 5, p. 10-pg., 2022-02-23. (17/22739-9)

TEIXEIRA, JORGE FELIPE LIMA; DE SOUZA, JOAO ANTONIO CHAVES; MAGALHAES, FERNANDO AUGUSTO CINTRA; DE OLIVEIRA, GUILHERME JOSE PIMENTEL LOPES; DE SANTIS, JOSE BERNARDO; COSTA, CARLOS ALBERTO DE SOUZA; DE SOUZA, PEDRO PAULO CHAVES. Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 14, n. 4, p. 12-pg., 2023-04-01. (14/05283-3, 17/22739-9)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; WU MON, FILIPE KOON; BORDINI, ESTER ALVES FERREIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Bioactivity effects of extracellular matrix proteins on apical papilla cells. Journal of Applied Oral Science, v. 29, 2021. (17/22739-9, 17/14210-8)

LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; MENDES SOARES, IGOR PAULINO; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Development of fibronectin-loaded nanofiber scaffolds for guided pulp tissue regeneration. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 9, p. 1244-1258, SEP 2021. (17/22739-9, 17/14210-8)

LEITE, MARIA LUISA; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; SOARES, DIANA GABRIELA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Poly(caprolactone)-aligned nanofibers associated with fibronectin-loaded collagen hydrogel as a potent bioactive scaffold for cell-free regenerative endodontics. International Endodontic Journal, v. 55, n. 12, p. 13-pg., 2022-09-10. (17/14210-8, 17/22739-9)