| Texto completo | |
| Autor(es): |
Lopes, Helena B.
[1]
;
Freitas, Gileade P.
[1]
;
Elias, Carlos N.
[2]
;
Tye, Coralee
[3]
;
Stein, Janet L.
[3]
;
Stein, Gary S.
[3]
;
Lian, Jane B.
[3]
;
Rosa, Adalberto L.
[1]
;
Beloti, Marcio M.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Cell Culture Lab, Sch Dent Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Inst Mil Engn, Biomat Lab, Rio De Janeiro, RJ - Brazil
[3] Univ Vermont, Dept Biochem, Canc Ctr, Burlington, VT 05405 - USA
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Biomedical Materials Research Part A; v. 107, n. 6, p. 1303-1313, JUN 2019. |
| Citações Web of Science: | 2 |
| Resumo | |
The major role of integrins is to mediate cell adhesion but some of them are involved in the osteoblasts-titanium (Ti) interactions. In this study, we investigated the participation of integrins in osteoblast differentiation induced by Ti with nanotopography (Ti-Nano) and with microtopography (Ti-Micro). By using a PCR array, we observed that, compared with Ti-Micro, Ti-Nano upregulated the expression of five integrins in mesenchymal stem cells, including integrin beta 3, which increases osteoblast differentiation. Silencing integrin beta 3, using CRISPR-Cas9, in MC3T3-E1 cells significantly reduced the osteoblast differentiation induced by Ti-Nano in contrast to the effect on T-Micro. Concomitantly, integrin beta 3 silencing downregulated the expression of integrin alpha v, the parent chain that combines with other integrins and several components of the Wnt/beta-catenin and BMP/Smad signaling pathways, all involved in osteoblast differentiation, only in cells cultured on Ti-Nano. Taken together, our results showed the key role of integrin beta 3 in the osteogenic potential of Ti-Nano but not of Ti-Micro. Additionally, we propose a novel mechanism to explain the higher osteoblast differentiation induced by Ti-Nano that involves an intricate regulatory network triggered by integrin beta 3 upregulation, which activates the Wnt and BMP signal transductions. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1303-1313, 2019. (AU) | |
| Processo FAPESP: | 13/05181-3 - Participação de integrinas na diferenciação osteoblástica de células-tronco mesenquimais induzida por superfícies de titânio com micro e nanotopografia |
| Beneficiário: | Márcio Mateus Beloti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/21116-5 - Uso do CRISPR-Cas9 na Investigação do Papel das Integrinas na Diferenciação Osteoblastica de Células-Tronco Mesenquimais Induzidas por Superfícies de Titânio |
| Beneficiário: | Helena Bacha Lopes |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 14/08443-1 - Participação de integrinas na diferenciação osteoblástica de células-tronco mesenquimais induzida por superfícies de titânio com micro e nanotopografia |
| Beneficiário: | Helena Bacha Lopes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |