Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo

Autor(es)

Castillo Pedraza, Midian C. ^[1] ; Rosalen, Pedro L. ^[2] ; Freire de Castilho, Aline Rogeria ^{[2, 3]} ; Freires, Irian de Almeida ^{[4, 2]} ; Leite, Luana de Sales ^[1] ; Faustoferri, Roberta C. ^[5] ; Quivey, Jr., Robert G. ^[5] ; Klein, I, Marlise

Afiliação do(s) autor(es)

^[1] I, Sao Paulo State Univ Unesp, Dept Dent Mat & Prosthodont, Sch Dent, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Estadual Campinas, Dept Physiol Sci, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil

^[3] Univ Estadual Campinas, Dept Pediat Dent, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil

^[4] Univ Florida, Coll Dent, Dept Oral Biol, Gainesville, FL 32610 - USA

^[5] Univ Rochester, Ctr Oral Biol, Rochester, NY - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL MICROBIOLOGY; v. 11, n. 1 JAN 1 2019.
• Citações Web of Science: 0

Resumo

Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (Delta lytS and Delta lytT), LTA (Delta dltA and Delta dltD), and insoluble exopolysaccharides (Delta gtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with increment lytS, increment dltD, and Delta gtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo. (AU)

• Processo FAPESP: 14/01723-9 - Caracterização de um cimento de ionômero de vidro contendo produtos naturais: estudos in vitro e in vivo
• Beneficiário: Aline Rogéria Freire de Castilho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/00753-0 - Análises in vivo da virulência dos genes lytTS e operon dltABCD de Streptococcus mutans e modulação dos mesmo por terapia direcionada
• Beneficiário: Midian Clara Castillo Pedraza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/05423-0 - Função de DNA extracelular e de ácidos lipoteicóicos na matriz extracelular de biofilmes cariogênicos
• Beneficiário: Marlise Inêz Klein Furlan
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/08021-5 - Análise da expressão de genes de Streptococcus mutans em biofilme misto
• Beneficiário: Luana de Sales Leite
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico