Mast Cell Protease 7 Promotes Angiogenesis by Degradation of Integrin Subunits

Autor(es)

de Souza Junior, Devandir A. ^[1] ; Santana, Carolina ^[1] ; Vieira, Gabriel V. ^[1] ; Oliver, Constance ^[1] ; Jamur, Maria Celia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, BR-14049900 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 8, n. 4 APR 2019.
• Citações Web of Science: 0

Resumo

Previous studies from our laboratory have shown that during angiogenesis in vitro, rmMCP-7 (recombinant mouse mast cell protease-7) stimulates endothelial cell spreading and induces their penetration into the matrix. The ability of rmMCP-7 to induce angiogenesis in vivo was assessed in the present study using a directed in vivo angiogenesis assay (DIVAA). Vessel invasion of the angioreactor was observed in the presence of rmMCP-7 but was not seen in the control. Since integrins are involved in endothelial cell migration, the relationship between rmMCP-7 and integrins during angiogenesis was investigated. Incubation with rmMCP-7 resulted in a reduction in the levels of integrin subunits v and 1 on SVEC4-10 endothelial cells during angiogenesis in vitro. Furthermore, the degradation of integrin subunits occurs both through the direct action of rmMCP-7 and indirectly via the ubiquitin/proteasome system. Even in the presence of a proteasome inhibitor, incubation of endothelial cells with rmMCP-7 induced cell migration and tube formation as well as the beginning of loop formation. These data indicate that the direct degradation of the integrin subunits by rmMCP-7 is sufficient to initiate angiogenesis. The results demonstrate, for the first time, that mMCP-7 acts in angiogenesis through integrin degradation. (AU)

• Processo FAPESP: 17/18618-1 - Interação entre mastócitos e células endoteliais durante a angiogênese in vitro
• Beneficiário: Constance Oliver
• Modalidade de apoio: Auxílio à Pesquisa - Regular