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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mast Cell Protease 7 Promotes Angiogenesis by Degradation of Integrin Subunits

Full text
Author(s):
de Souza Junior, Devandir A. [1] ; Santana, Carolina [1] ; Vieira, Gabriel V. [1] ; Oliver, Constance [1] ; Jamur, Maria Celia [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 1
Document type: Journal article
Source: CELLS; v. 8, n. 4 APR 2019.
Web of Science Citations: 0
Abstract

Previous studies from our laboratory have shown that during angiogenesis in vitro, rmMCP-7 (recombinant mouse mast cell protease-7) stimulates endothelial cell spreading and induces their penetration into the matrix. The ability of rmMCP-7 to induce angiogenesis in vivo was assessed in the present study using a directed in vivo angiogenesis assay (DIVAA). Vessel invasion of the angioreactor was observed in the presence of rmMCP-7 but was not seen in the control. Since integrins are involved in endothelial cell migration, the relationship between rmMCP-7 and integrins during angiogenesis was investigated. Incubation with rmMCP-7 resulted in a reduction in the levels of integrin subunits v and 1 on SVEC4-10 endothelial cells during angiogenesis in vitro. Furthermore, the degradation of integrin subunits occurs both through the direct action of rmMCP-7 and indirectly via the ubiquitin/proteasome system. Even in the presence of a proteasome inhibitor, incubation of endothelial cells with rmMCP-7 induced cell migration and tube formation as well as the beginning of loop formation. These data indicate that the direct degradation of the integrin subunits by rmMCP-7 is sufficient to initiate angiogenesis. The results demonstrate, for the first time, that mMCP-7 acts in angiogenesis through integrin degradation. (AU)

FAPESP's process: 17/18618-1 - Interaction between mast cells and endothelial cells during in vitro angiogenesis
Grantee:Constance Oliver
Support Opportunities: Regular Research Grants