Maternal apical periodontitis is associated with insulin resistance in adult offspring

Autor(es)

Tsosura, T. V. S. ^[1] ; Chiba, F. Y. ^[2] ; Mattera, M. S. L. C. ^[1] ; Pereira, R. F. ^[1] ; Cintra, L. T. A. ^[3] ; Conti, L. C. ^[3] ; dos Santos, R. M. ^[1] ; Mateus, J. H. P. ^[1] ; Garbin, C. A. S. ^[2] ; Sumida, D. H. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, SBFis, Multictr Postgrad Program Physiol Sci, Dept Basic Sci, Aracatuba - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Children & Social Dent, Aracatuba - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Restorat Dent, Sch Dent, Aracatuba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 52, n. 7, p. 1040-1050, JUL 2019.
• Citações Web of Science: 0

Resumo

Aim To investigate the plasma concentrations of glucose, insulin and tumour necrosis factor-alpha (TNF-alpha) of rats with maternal apical periodontitis (AP) and to explore the effect of maternal inflammation on the initial steps of insulin signalling and the inflammatory pathway in the gastrocnemius muscle (GM) and periepididymal white adipose tissue (pWAT) of adult offspring. Methodology Fifteen female Wistar rats were distributed into a control group (CN), a group with 1 tooth with AP (1AP) and a group with 4 teeth with AP (4AP). Thirty days following induction of AP, female rats from all groups were mated with healthy male rats. When male offspring reached 75 days of age, plasma concentrations of glucose, insulin and TNF-alpha were quantified. Insulin resistance was evaluated by the homoeostasis model assessment of insulin resistance (HOMA-IR) index. Phosphorylation status of pp185 tyrosine, insulin receptor substrate 1 (IRS-1) serine, I kappa B kinase alpha/beta (IKK alpha/beta) and c-Jun N-terminal kinase (JNK) in the GM and pWAT were measured by Western blot. Analysis of variance was performed, followed by the Tukey's post hoc test. P values Maternal AP promoted insulin resistance, impaired the initial steps of insulin signalling, significantly increased plasma concentrations of insulin (P < 0.001) and TNF-alpha (P < 0.05), and enhanced IKK alpha/beta phosphorylation in the GM and pWAT (P < 0.05) of adult offspring. However, maternal AP did not affect fasting glycaemia and JNK phosphorylation in the GM and pWAT of adult offspring. Conclusions Maternal AP was associated with insulin resistance in adult offspring through alterations in insulin signalling and inflammation pathways. The study provides information on the impact of maternal AP on the development of metabolic alterations such as insulin resistance in adult offspring and reinforces the importance of preventing maternal AP in order to maintain the general health of offspring. (AU)

• Processo FAPESP: 14/26517-2 - Avaliação do conteúdo da proteína transportadora de glicose GLUT4 em músculo esquelético de ratos adultos com lesão periapical
• Beneficiário: Renan Akira Fujii de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/24829-2 - Avaliação da infiltração de macrófagos, do sinal inflamatório e atrofia em músculo esquelético de ratos com lesão periapical
• Beneficiário: Doris Hissako Matsushita
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/01128-1 - Avaliação das vias insulínica e inflamatória em tecidos muscular esquelético e adiposo de ratos adultos, proles de ratas com lesão periapical
• Beneficiário: Thaís Verônica Saori Tsosura
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/18397-5 - Avaliação do sinal insulínico em tecido muscular esquelético de ratos adultos, proles de ratas com lesão periapical
• Beneficiário: Jhenifer Haiane Pereira Mateus
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica