Maternal apical periodontitis is associated with insulin resistance in adult offspring

Author(s)

Tsosura, T. V. S. ^[1] ; Chiba, F. Y. ^[2] ; Mattera, M. S. L. C. ^[1] ; Pereira, R. F. ^[1] ; Cintra, L. T. A. ^[3] ; Conti, L. C. ^[3] ; dos Santos, R. M. ^[1] ; Mateus, J. H. P. ^[1] ; Garbin, C. A. S. ^[2] ; Sumida, D. H. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, SBFis, Multictr Postgrad Program Physiol Sci, Dept Basic Sci, Aracatuba - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Children & Social Dent, Aracatuba - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Restorat Dent, Sch Dent, Aracatuba - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 52, n. 7, p. 1040-1050, JUL 2019.
• Web of Science Citations: 0

Abstract

Aim To investigate the plasma concentrations of glucose, insulin and tumour necrosis factor-alpha (TNF-alpha) of rats with maternal apical periodontitis (AP) and to explore the effect of maternal inflammation on the initial steps of insulin signalling and the inflammatory pathway in the gastrocnemius muscle (GM) and periepididymal white adipose tissue (pWAT) of adult offspring. Methodology Fifteen female Wistar rats were distributed into a control group (CN), a group with 1 tooth with AP (1AP) and a group with 4 teeth with AP (4AP). Thirty days following induction of AP, female rats from all groups were mated with healthy male rats. When male offspring reached 75 days of age, plasma concentrations of glucose, insulin and TNF-alpha were quantified. Insulin resistance was evaluated by the homoeostasis model assessment of insulin resistance (HOMA-IR) index. Phosphorylation status of pp185 tyrosine, insulin receptor substrate 1 (IRS-1) serine, I kappa B kinase alpha/beta (IKK alpha/beta) and c-Jun N-terminal kinase (JNK) in the GM and pWAT were measured by Western blot. Analysis of variance was performed, followed by the Tukey's post hoc test. P values Maternal AP promoted insulin resistance, impaired the initial steps of insulin signalling, significantly increased plasma concentrations of insulin (P < 0.001) and TNF-alpha (P < 0.05), and enhanced IKK alpha/beta phosphorylation in the GM and pWAT (P < 0.05) of adult offspring. However, maternal AP did not affect fasting glycaemia and JNK phosphorylation in the GM and pWAT of adult offspring. Conclusions Maternal AP was associated with insulin resistance in adult offspring through alterations in insulin signalling and inflammation pathways. The study provides information on the impact of maternal AP on the development of metabolic alterations such as insulin resistance in adult offspring and reinforces the importance of preventing maternal AP in order to maintain the general health of offspring. (AU)

• FAPESP's process: 14/26517-2 - Assessment of the glucose transporter protein GLUT4 content in skeletal muscle of adult rats with periapical lesion
• Grantee: Renan Akira Fujii de Oliveira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/24829-2 - Evaluation of macrophages infiltration, inflammatory signal and skeletal muscle atrophy of rats with periapical lesion
• Grantee: Doris Hissako Matsushita
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/01128-1 - Assessment of insulin signaling and inflammatory pathways in skeletal muscle and adipose tissues of adult rats, offspring of the rats with periapical lesion
• Grantee: Thaís Verônica Saori Tsosura
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/18397-5 - Assessment of the insulin signaling in skeletal muscle tissue of adult rats, offspring of rats with periapical lesion
• Grantee: Jhenifer Haiane Pereira Mateus
• Support Opportunities: Scholarships in Brazil - Scientific Initiation