Enhancement of lipase transesterification activity by immobilization on -cyclodextrin-based polymer

Autor(es)

Martin, Lucas S. ^{[1, 2]} ; Ceron, Annie A. ^[1] ; Molinari, Deise ^[2] ; De Moraes, Flavio F. ^[2] ; Arroyo, Pedro A. ^[2] ; De Castro, Heizir F. ^[1] ; Zanin, Gisella M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Engn Sch Lorena, BR-12602810 Lorena, SP - Brazil

^[2] Univ Estadual Maringa, Chem Engn Dept, BR-87020900 Maringa, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Sol-Gel Science and Technology; v. 91, n. 1, p. 92-100, JUL 2019.
• Citações Web of Science: 0

Resumo

Silica--cyclodextrin matrices were prepared by the sol-gel technique using tetraethoxysilane as precursor and different concentrations (0.5-4.0%) of the organic component, -cyclodextrin (-CD). Hybrid matrices were activated with glutaraldehyde to introduce functional groups for the attachment of Burkholderia cepacia lipase, used here as a model enzyme. The goal was to produce biocatalysts with high activity and satisfactory properties for transesterification reactions. The biocatalysts exhibited similar hydrolytic activities, coupling yields, and kinetic parameters (K-m and V-max), but differed in terms of thermal stability and transesterification activity. The best biocatalyst, prepared using 2.0% -CD, was used to study the influence of oil/alcohol molar ratio on ester yield, viscosity, and residual monoacylglycerol (MG) and diacylglycerol (DG) contents. The molar ratio of palm kernel oil to ethanol had a considerable effect on the formation of ethyl esters and the best performance was achieved with an oil/alcohol molar ratio of 1:6. Under this condition, a high ethyl ester yield, a low residual MG content (<0.5%), and undetectable DG levels were obtained. Kinematic viscosity measurements (40 degrees C) confirmed the high transesterification yield, as the viscosity of palm kernel oil decreased from 30.13mm(2)s(-1) to <4mm(2)s(-1) {[}GRAPHICS] . A supported immobilized lipase was investigated as a suitable biocatalyst for the transesterification of palm kernel oil to ethyl esters. The biocatalyst compromises a non-specific lipase from Burkholderia cepacia immobilized on silica--cyclodextrin matrix prepared by the sol-gel technique using tetraethoxysilane as a precursor and 2.0% of the organic component, -cyclodextrin (-CD). HighlightsSilica--cyclodextrin matrices were prepared by the sol-gel technique using different ratios of inorganic/organic compounds.The matrices showed similar properties although affect the biocatalyst performance.The best set of parameters was achieved on lipase immobilized on matrix prepared with 2.0% -CD.The most active biocatalyst was successful applied in the transesterification of palm kernel oil. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático