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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enhancement of lipase transesterification activity by immobilization on -cyclodextrin-based polymer

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Author(s):
Martin, Lucas S. [1, 2] ; Ceron, Annie A. [1] ; Molinari, Deise [2] ; De Moraes, Flavio F. [2] ; Arroyo, Pedro A. [2] ; De Castro, Heizir F. [1] ; Zanin, Gisella M. [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Engn Sch Lorena, BR-12602810 Lorena, SP - Brazil
[2] Univ Estadual Maringa, Chem Engn Dept, BR-87020900 Maringa, PR - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Sol-Gel Science and Technology; v. 91, n. 1, p. 92-100, JUL 2019.
Web of Science Citations: 0
Abstract

Silica--cyclodextrin matrices were prepared by the sol-gel technique using tetraethoxysilane as precursor and different concentrations (0.5-4.0%) of the organic component, -cyclodextrin (-CD). Hybrid matrices were activated with glutaraldehyde to introduce functional groups for the attachment of Burkholderia cepacia lipase, used here as a model enzyme. The goal was to produce biocatalysts with high activity and satisfactory properties for transesterification reactions. The biocatalysts exhibited similar hydrolytic activities, coupling yields, and kinetic parameters (K-m and V-max), but differed in terms of thermal stability and transesterification activity. The best biocatalyst, prepared using 2.0% -CD, was used to study the influence of oil/alcohol molar ratio on ester yield, viscosity, and residual monoacylglycerol (MG) and diacylglycerol (DG) contents. The molar ratio of palm kernel oil to ethanol had a considerable effect on the formation of ethyl esters and the best performance was achieved with an oil/alcohol molar ratio of 1:6. Under this condition, a high ethyl ester yield, a low residual MG content (<0.5%), and undetectable DG levels were obtained. Kinematic viscosity measurements (40 degrees C) confirmed the high transesterification yield, as the viscosity of palm kernel oil decreased from 30.13mm(2)s(-1) to <4mm(2)s(-1) {[}GRAPHICS] . A supported immobilized lipase was investigated as a suitable biocatalyst for the transesterification of palm kernel oil to ethyl esters. The biocatalyst compromises a non-specific lipase from Burkholderia cepacia immobilized on silica--cyclodextrin matrix prepared by the sol-gel technique using tetraethoxysilane as a precursor and 2.0% of the organic component, -cyclodextrin (-CD). HighlightsSilica--cyclodextrin matrices were prepared by the sol-gel technique using different ratios of inorganic/organic compounds.The matrices showed similar properties although affect the biocatalyst performance.The best set of parameters was achieved on lipase immobilized on matrix prepared with 2.0% -CD.The most active biocatalyst was successful applied in the transesterification of palm kernel oil. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants