Emollient esters synthesis catalyzed by immobilized lipase on styrene-based polymers with magnetic properties

• Grant number: 16/17833-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: July 31, 2019
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Larissa de Freitas
• Grantee: Larissa de Freitas
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Associated researchers: Leandro Gonçalves de Aguiar ; Patricia Caroline Molgero Da Rós ; Pedro Carlos de Oliveira

Abstract

The present project aims to synthesize emollient esters, which are very often used in cosmetic formulations due to acting as moisturizing agents and balancing skin oiliness, with microbial lipase immobilization on styrene-based copolymers that were magnetized by co-precipitation of Fe+2and Fe+3 ions as catalysts. Its development is aimed at strengthening a line of research in the Department of Chemical Engineering at the Engineering School of Lorena (EEL). The selected proposal for producing the present work comprises the development of magnetically active and stable biocatalysts which are going to be employed in the production of an important class of carboxylic esters, aiming at its implementation in the cosmetic sector, especially for cosmetic formulations in skincare. For such a purpose, it will be firstly established optimal conditions of magnetic copolymer synthesis in order to obtain a hybrid support with appropriate characteristics for further microbial lipase immobilization. It will be conducted by physical adsorption with which different methods for drying the biocatalyst are going to be studied. Both the morphological characterization of the synthesized supports and the determination of biochemical and kinetic properties of the obtained immobilized derivatives are going to be conducted. Afterwards, it is going to be evaluated the performance of different systems being immobilized by esterification reactions in a solvent medium with the purpose of synthesizing long-chain esters which act as emollients in cosmetic industries. As a result, a comprehensive analysis of reaction parameters is going to be carried out in order to select the best operational conditions for the bioprocess by the design of experiments, aiming at a possible application in continuous processes. In this way, the contribution of the present project is the development of national technologies of enzymatic processes as viable alternatives to conventional chemical processes in order to manufacture products of industrial, economic, environmental and social interest. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILAS BOAS, RENATA N.; LIMA, ROSEMAR; SILVA, MATEUS V. C.; FREITAS, LARISSA; AGUIAR, LEANDRO G.; DE CASTRO, HEIZIR F.. Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor. Bioprocess and Biosystems Engineering, JUN 2021. (16/10636-8, 16/17833-3)

DA SILVA, MATEUS V. C.; SOUZA, AMANDA B.; DE CASTRO, HEIZIR F.; AGUIAR, LEANDRO G.; DE OLIVEIRA, PEDRO C.; DE FREITAS, LARISSA. Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow. Bioprocess and Biosystems Engineering, v. 43, n. 4, p. 615-623, APR 2020. (17/04934-9, 16/17833-3, 16/10636-8)

ROSA, CINTIA MARIA RODRIGUES; CASAGRANDE DA SILVA, MATEUS VINICIUS; DE AGUIAR, LEANDRO GONCALVES; DE CASTRO, HEIZIR FERREIRA; FREITAS, LARISSA. Prediction and comparison of textural properties of magnetic copolymer supports for enzyme immobilization. Journal of Applied Polymer Science, v. 137, n. 41, MAR 2020. (17/04934-9, 16/17833-3, 16/10636-8)

DA SILVA, MATEUS V. C.; RANGEL, AMANDA B. S.; DUTRA, FELLIPE P.; DE CASTRO, HEIZIR F.; DE FREITAS, LARISSA. Kinetic and Thermodynamic Study of 2-Ethylhexyl Oleate Synthesis Catalyzed by Candida antarctica Lipase Immobilized on a Magnetic Hybrid Support. CATALYSIS LETTERS, v. 151, n. 5, p. 1239-1247, MAY 2021. (16/10636-8, 16/17833-3)

SILVA, MATEUS V. C.; ROSA, CINTIA M. R.; AGUIAR, LEANDRO G.; OLIVEIRA, PEDRO C.; DE CASTRO, HEIZIR F.; FREITAS, LARISSA. Synthesis of Isopropyl Palmitate by Lipase Immobilized on a Magnetized Polymer Matrix. CHEMICAL ENGINEERING & TECHNOLOGY, v. 43, n. 9, JUN 2020. (16/10636-8, 16/17833-3)

DA SILVA, MATEUS V. C.; RANGEL, AMANDA B. S.; ROSA, CINTIA M. R.; DE ASSIS, GABRIELLE P.; AGUIAR, LEANDRO G.; DE FREITAS, LARISSA. Development of a magnetically stabilized fluidized bed bioreactor for enzymatic synthesis of 2-ethylhexyl oleate. Bioprocess and Biosystems Engineering, v. N/A, p. 12-pg., 2023-10-10. (16/10636-8, 16/17833-3)

RANGEL, AMANDA B. S.; SILVA, MATEUS V. C.; DE ASSIS, GABRIELLE P. P.; ROSA, CINTIA M. R.; DOS SANTOS, JULIO C.; DE FREITAS, LARISSA. Synthesis and Characterization of Magnetic Poly(STY-EGDMA) Particles for Application as Biocatalyst Support in Octyl Oleate Ester Synthesis: Kinetic and Thermodynamic Parameters and Mathematical Modeling. CATALYSIS LETTERS, v. N/A, p. 13-pg., 2022-12-08. (16/10636-8, 16/17833-3)

DA SILVA, MATEUS V. C.; RANGEL, AMANDA B. S.; AGUIAR, LEANDRO G.; DE CASTRO, HEIZIR F.; DE FREITAS, LARISSA. Continuous Enzymatic Synthesis of 2-Ethylhexyl Oleate in a Fluidized Bed Reactor: Operating Conditions, Hydrodynamics, and Mathematical Modeling. Industrial & Engineering Chemistry Research, v. 59, n. 44, p. 19522-19530, NOV 4 2020. (16/10636-8, 16/17833-3)

SILVA, MATEUS V. C.; AGUIAR, LEANDRO G.; DE CASTRO, HEIZIR F.; FREITAS, LARISSA. Optimization of the parameters that affect the synthesis of magnetic copolymer styrene-divinilbezene to be used as efficient matrix for immobilizing lipases. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 34, n. 11, NOV 2018. (16/17833-3, 16/10636-8)

VILAS BOAS, RENATA N.; LIMA, ROSEMAR; SILVA, MATEUS V. C.; FREITAS, LARISSA; AGUIAR, LEANDRO G.; DE CASTRO, HEIZIR F.. Continuous production of monoacylglycerol via glycerolysis of babassu oil by immobilized Burkholderia cepacia lipase in a packed bed reactor. Bioprocess and Biosystems Engineering, v. 44, n. 10, p. 2205-2215, OCT 2021. (16/10636-8, 16/17833-3)