Blockage of the P2X7 Receptor Attenuates Harmful Changes Produced by Ischemia and Reperfusion in the Myenteric Plexus

Autor(es)

Palombit, Kelly ^{[1, 2]} ; Mendes, Cristina Eusebio ^[1] ; Tavares-de-Lima, Wothan ^[3] ; Barreto-Chaves, Maria Luiza ^[1] ; Castelucci, Patricia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, Ave Prof Dr Lineu Prestes, 2415, BR-05508900 Sao Paulo - Brazil

^[2] Univ Fed Piaui, Dept Morphol, Teresina - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Digestive Diseases and Sciences; v. 64, n. 7, p. 1815-1829, JUL 2019.
• Citações Web of Science: 0

Resumo

IntroductionOur work analyzed the effects of a P2X7 receptor antagonist, Brilliant Blue G (BBG), on rat ileum myenteric plexus following ischemia and reperfusion (ISR) induced by 45min of ileal artery occlusion with an atraumatic vascular clamp with 24h (ISR 24-h group) or 14 d of reperfusion (ISR 14-d group).Material and methodsEither BBG (50mg/kg or 100mg/kg, BBG50 or BBG100 groups) or saline (vehicle) was administered subcutaneously 1h after ischemia in the ISR 24-h group or once daily for the 5 d after ischemia in the ISR 14-d group (n=5 per group). We evaluated the neuronal density and profile area by examining the number of neutrophils in the intestinal layers, protein expression levels of the P2X7 receptor, intestinal motility and immunoreactivity for the P2X7 receptor, nitric oxide synthase, neurofilament-200, and choline acetyl transferase in myenteric neurons.ResultsThe neuronal density and profile area were restored by BBG following ISR. The ischemic groups showed alterations in P2X7 receptor protein expression and the number of neutrophils in the intestine and decreased intestinal motility, all of which were recovered by BBG treatment.ConclusionWe concluded that ISR morphologically and functionally affected the intestine and that its effects were reversed by BBG treatment, suggesting the P2X7 receptor as a therapeutic target. (AU)

• Processo FAPESP: 10/10510-8 - Estudo do efeito da isquemia e reperfusão intestinal sobre o receptor purina p2x7 e o sistema nervoso entérico do íleo de ratos
• Beneficiário: Kelly Palombit
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/00259-1 - Papel do antagonista brilliant blue green (BBG) sobre o receptor P2X7 e o sistema nervoso entérico do íleo de ratos submetidos a isquemia e reperfusão intestinal: estudo morfofuncional
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/25927-2 - Aspectos morfológicos, moleculares e funcionais da interação entre o receptor P2X7 e a panexina-1 nas células gliais entéricas após a isquemia/reperfusão intestinal
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Auxílio à Pesquisa - Regular