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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Blockage of the P2X7 Receptor Attenuates Harmful Changes Produced by Ischemia and Reperfusion in the Myenteric Plexus

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Author(s):
Palombit, Kelly [1, 2] ; Mendes, Cristina Eusebio [1] ; Tavares-de-Lima, Wothan [3] ; Barreto-Chaves, Maria Luiza [1] ; Castelucci, Patricia [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, Ave Prof Dr Lineu Prestes, 2415, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Piaui, Dept Morphol, Teresina - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Digestive Diseases and Sciences; v. 64, n. 7, p. 1815-1829, JUL 2019.
Web of Science Citations: 0
Abstract

IntroductionOur work analyzed the effects of a P2X7 receptor antagonist, Brilliant Blue G (BBG), on rat ileum myenteric plexus following ischemia and reperfusion (ISR) induced by 45min of ileal artery occlusion with an atraumatic vascular clamp with 24h (ISR 24-h group) or 14 d of reperfusion (ISR 14-d group).Material and methodsEither BBG (50mg/kg or 100mg/kg, BBG50 or BBG100 groups) or saline (vehicle) was administered subcutaneously 1h after ischemia in the ISR 24-h group or once daily for the 5 d after ischemia in the ISR 14-d group (n=5 per group). We evaluated the neuronal density and profile area by examining the number of neutrophils in the intestinal layers, protein expression levels of the P2X7 receptor, intestinal motility and immunoreactivity for the P2X7 receptor, nitric oxide synthase, neurofilament-200, and choline acetyl transferase in myenteric neurons.ResultsThe neuronal density and profile area were restored by BBG following ISR. The ischemic groups showed alterations in P2X7 receptor protein expression and the number of neutrophils in the intestine and decreased intestinal motility, all of which were recovered by BBG treatment.ConclusionWe concluded that ISR morphologically and functionally affected the intestine and that its effects were reversed by BBG treatment, suggesting the P2X7 receptor as a therapeutic target. (AU)

FAPESP's process: 10/10510-8 - STUDY OF EFFECT OF THE INTESTINAL ISCHEMIA AND REPERFUSION ON PURINE P2X7 RECEPTOR AND ENTERIC NERVOUS SYSTEM OF THE RATS ILEUM
Grantee:Kelly Palombit
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/00259-1 - Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study
Grantee:Patricia Castelucci
Support Opportunities: Regular Research Grants
FAPESP's process: 14/25927-2 - Morphological, molecular and functional aspects of the interaction between the P2X7 receptor and pannexin-1 in the enteric glial cells following intestinal ischemia/reperfusion
Grantee:Patricia Castelucci
Support Opportunities: Regular Research Grants