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Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study

Grant number: 12/00259-1
Support type:Regular Research Grants
Duration: May 01, 2012 - October 31, 2014
Field of knowledge:Biological Sciences - Morphology
Principal researcher:Patricia Castelucci
Grantee:Patricia Castelucci
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Abdominal pain can be consequent to several causes, among the various possibilities can be intestinal ischemia. In the digestive tract ischemia / reperfusion (I / Ri) causes morphological changes in enteric neurons. This work aims to analyze the effect of the antagonist Brilliant Blue Green (BBG) and the P2X7 receptor on the enteric nervous system of the ileum of rats subjected to I / Ri (ischemia and reperfusion). The intestinal ischemia will be obtained by the obstruction of blood flow ileal arteries by the time of 45 minutes followed by reperfusion for 4, 24 hours and 14 days. There is a group without reperfusion. The sham group means no occlusion of the ileal arteries. The P2X7 receptor antagonist, BBG is injected 1 hour after ischemia in the group of 24 h, and for 5 consecutive days in the group of 14 days. The tissue will be prepared by methods of double and triple immunohistochemical markers P2X7 receptor with Nitric Oxide Synthase (NOS), neurofilament (NF), acetylcholine transferase (CHAT), calretinin (CALR), Neuropeptide Y (NPY) and Vasoactive Intestinal Peptide (VIP) and the pan-neuronal anti-HuC / D. For qualitative and quantitative analysis will be used fluorescence microscopy and Confocal Laser Scanning. Other techniques will be used for analysis: the P2X7 receptor protein expression by Western blotting, the presence of neutrophils by histochemical technique of myeloperoxidase (MPO), apoptosis by TUNNEL Technical and functional activity of intestinal motility, the technique will be by organ bath. After, there will be statistical analysis of group data. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PALOMBIT, KELLY; MENDES, CRISTINA EUSEBIO; TAVARES-DE-LIMA, WOTHAN; BARRETO-CHAVES, MARIA LUIZA; CASTELUCCI, PATRICIA. Blockage of the P2X7 Receptor Attenuates Harmful Changes Produced by Ischemia and Reperfusion in the Myenteric Plexus. Digestive Diseases and Sciences, v. 64, n. 7, p. 1815-1829, . (10/10510-8, 12/00259-1, 14/25927-2)
SCHAFER, BARBARA TAVARES; SILVEIRA, MARIANA POVOA; PALOMBIT, KELLY; MENDES, CRISTINA EUSEBIO; WATANABE, II SEI; MIGLINO, MARIA ANGELICA; CASTELUCCI, PATRICIA. Morphological Characterization of the Myenteric Plexus of the Ileum and Distal colon of Dogs Affected by Muscular Dystrophy. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 301, n. 4, p. 673-685, . (12/00259-1, 08/05314-5)
EVANGELINELLIS, MARIA MUNHOZ; SOUZA, ROBERTA FIGUEIROA; MENDES, CRISTINA EUSEBIO; CASTELUCCI, PATRICIA. Effects of a P2X7 receptor antagonist on myenteric neurons in the distal colon of an experimental rat model of ulcerative colitis. Histochemistry and Cell Biology, v. 157, n. 1, . (18/07862-1, 12/00259-1, 14/25927-2)
MENDES, CRISTINA EUSEBIO; PALOMBIT, KELLY; VIEIRA, CATIA; SILVA, ISABEL; CORREIA-DE-SA, PAULO; CASTELUCCI, PATRICIA. The Effect of Ischemia and Reperfusion on Enteric Glial Cells and Contractile Activity in the Ileum. Digestive Diseases and Sciences, v. 60, n. 9, p. 2677-2689, . (08/08728-5, 12/00259-1, 08/05314-5, 12/06434-0)
DA SILVA, MARCOS VINICIUS; MAROSTI, ALINE ROSA; MENDES, CRISTINA EUSEBIO; PALOMBIT, KELLY; CASTELUCCI, PATRICIA. Submucosal neurons and enteric glial cells expressing the P2X7 receptor in rat experimental colitis. ACTA HISTOCHEMICA, v. 119, n. 5, p. 481-494, . (12/00259-1, 08/05314-5)
MENDES, CRISTINA EUSEBIO; PALOMBIT, KELLY; TAVARES-DE-LIMA, WOTHAN; CASTELUCCI, PATRICIA. Enteric glial cells immunoreactive for P2X7 receptor are affected in the ileum following ischemia and reperfusion. ACTA HISTOCHEMICA, v. 121, n. 6, p. 665-679, . (12/00259-1, 14/25927-2)

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