The Effect of Ischemia and Reperfusion on Enteric Glial Cells and Contractile Activity in the Ileum

Author(s)

Mendes, Cristina Eusebio ^[1] ; Palombit, Kelly ^[1] ; Vieira, Catia ^[2] ; Silva, Isabel ^[2] ; Correia-de-Sa, Paulo ^[2] ; Castelucci, Patricia ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] ICBAS UP, Lab Pharmacol & Neurobiol, Ctr Drug Discovery & Innovat Med MedInUP, Oporto - Portugal

• Document type: Journal article
• Source: Digestive Diseases and Sciences; v. 60, n. 9, p. 2677-2689, SEP 2015.
• Web of Science Citations: 6

Abstract

We investigated the effects of ischemia followed by different periods of reperfusion (I/R) on immunoreactive S100 beta-positive glial and Hu-immunoreactive neurons co-expressing the P2X2 receptor in the myenteric plexus of the rat ileum. The ileal artery was occluded for 35 min with an atraumatic vascular clamp. The animals were killed 24 h, 72 h, and 1 week after ischemia. Sham animals were not submitted to ileal artery occlusion. The relative density, size, and co-localization of P2X2 receptor-expressing cells in relation to S100 beta-immunoreactive glial and Hu-immunoreactive neuronal cells were evaluated. Additionally, we analyzed the effects of I/R on gastrointestinal transit and ileum contractile activity. The cellular density of P2X2 receptor and neuronal Hu immunoreactivity/cm(2) decreased after I/R, whereas glial S100 beta immunoreactivity/cm(2) increased. No significant differences between sham and I/R groups were observed regarding the perikarya area of Hu-positive neurons. The area of S100 beta-immunoreactive glial cells increased by 24.1 % 1 week after I/R compared with the 24 h group. Methylene blue progression along the small intestine decreased (P < 0.05) from 24.5 +/- A 2.3 % in the sham group to 17.2 +/- A 2.0 % 1 week post-ischemia. We noted a significant (P < 0.05) decrease in the maximal contraction amplitude triggered by electrical field stimulation in the presence of ATP in preparations submitted to 24 h of I/R. Changes in the P2X2 receptor density parallel myenteric neuronal loss following I/R of the rat ileum. This, together with the increase in the activated (oversized) glial cells, may contribute to decreased GI motility after I/R. (AU)

• FAPESP's process: 08/08728-5 - Study of colocalization of the P2X2 e 7 receptors in the neurons of ileum enteric nervous system and enteric glial cells submitted to intestinal ischemia-reperfusion
• Grantee: Cristina Eusébio Mendes
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/00259-1 - Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study
• Grantee: Patricia Castelucci
• Support type: Regular Research Grants
• FAPESP's process: 08/05314-5 - Study of the chemical coding and P2X2 and P2X7 purina receptors of the ileum enteric nervous system of rats submitted to ischemia/reperfusion intestinaL of the ileal artery
• Grantee: Patricia Castelucci
• Support type: Regular Research Grants
• FAPESP's process: 12/06434-0 - Study of P2X7 receptor in the myenteric sinaptic transmission after ischemia and reperfusion of the ileum
• Grantee: Cristina Eusébio Mendes
• Support type: Scholarships abroad - Research Internship - Master's degree