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Study of colocalization of the P2X2 e 7 receptors in the neurons of ileum enteric nervous system and enteric glial cells submitted to intestinal ischemia-reperfusion

Grant number: 08/08728-5
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2009
Effective date (End): December 31, 2010
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal researcher:Patricia Castelucci
Grantee:Cristina Eusébio Mendes
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


ATP is known as a neurotransmitter and their receptors are the family of P2X1-7 (ABBRACHIO AND BURNSTOCK, 1998) and was shown the presence of P2X2 -3 receptor in the enteric nervous system (CASTELUCCI et al., 2002, et POOLE al., 2002; CASTELUCCI et al., 2003). In the gastrointestinal tract the ischemia /reperfusion intestinal causes morphological changes in enteric neurons (LINDESTRÖM and EKBLAD, 2004), with ischemia/reperfusion is observed in intestinal diseases such as the acute ischemia mesentery and Crohn's disease presenting as the processes of the necrosis in the intestine. This project aims to study the enteric nervous system of the ileum of rats submitted to ischemia / reperfusion intestinal of the ileal artery: a) distribution of P2X2 and P2X7 receptors, b) the proportion of neurons immunoreactive to anti HuC / D (neuronal marker) and coexpression with P2X2 and P2X7 receptors immunoreactivy neurons, c) the proportion of enteric gliais cells immunoreactive to tS-100 and that coexpression P2X2 and P2X7 receptor, d) the profiles and cell neuronal density of (neuron/mm2) immunoreactive to P2X2 and P2X7 receptors and anti HuC / D and glial cells. They will be examined ileum of the rats (n = 4 in ischemic and controls = 4/group) submitted to ischemia for 30 minutes and 2, 12, 24, 72 hours, 7 days and 1 month of reperfusion. Tissues will be prepared by immunohistochemical methods of simple and double markings of the receptor P2X2 and P2X7 and neuronal marker (anti-HuC / D) and label the cells gliais (S-100). The qualitative and quantitative analysis of counts of colocalizations, densities and profiles of neuronal and gliais be obtained from the fluorescence and Confocal scanning of the laser microscopes. After, statistical analyses will be made.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MENDES, CRISTINA EUSEBIO; PALOMBIT, KELLY; VIEIRA, CATIA; SILVA, ISABEL; CORREIA-DE-SA, PAULO; CASTELUCCI, PATRICIA. The Effect of Ischemia and Reperfusion on Enteric Glial Cells and Contractile Activity in the Ileum. Digestive Diseases and Sciences, v. 60, n. 9, p. 2677-2689, . (08/08728-5, 12/00259-1, 08/05314-5, 12/06434-0)

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