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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Morphological Characterization of the Myenteric Plexus of the Ileum and Distal colon of Dogs Affected by Muscular Dystrophy

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Schafer, Barbara Tavares [1] ; Silveira, Mariana Povoa [1] ; Palombit, Kelly [2, 3] ; Mendes, Cristina Eusebio [2] ; Watanabe, Ii Sei [2] ; Miglino, Maria Angelica [1] ; Castelucci, Patricia [2]
Total Authors: 7
[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Anat, Inst Biomed Sci, R Dr Lineu Prestes 2415, BR-05508900 Sao Paulo - Brazil
[3] Univ Fed Piaui, Deparment Morphol, Teresina, PI - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 301, n. 4, p. 673-685, APR 2018.
Web of Science Citations: 1

Duchenne-like muscular dystrophy (canine dystrophinopathy) is a hereditary degenerative disease characterized by muscle changes similar to those described for Duchenne muscular dystrophy (DMD) and by alterations in the smooth muscles of the gastrointestinal tract. Some authors have suggested that these abnormalities may be associated with intestinal motility. This study analyzed the nitrergic and cholinergic neurons and P2X7 receptor expression in the myenteric plexus of the ileum and distal colon of dogs with muscular dystrophy. Immunohistochemical techniques were used to detect nitric oxide synthase (NOS) and acetylcholine transferase (ChAT) expression and to label all HuC/D- and P2X7 receptor-immunoreactive (IR) neurons. Transmission electron microscopy and basic histology were performed for further analysis. The results showed that nitrergic neurons exhibited a Dogiel type I morphology in the ileum and distal colon. The neuronal profile results showed that there were fewer NOS-, ChAT-, and HuC/D-IR neurons in the ileum than in the distal colon in the dystrophic (DT) dogs. Additionally, there were more NOS-, ChAT- and HuC/D-IR neurons per ganglion in the distal colon than in the ileum. The P2X7 receptor-expressing neurons colocalized with nitrergic and cholinergic neurons. Transmission and light microscopy revealed collagen between the muscle fibers, between the circular and longitudinal muscle layers and within the myenteric ganglia of dogs with muscular dystrophy. These findings provide a morphological description of the myenteric neurons in the ileum and distal colon of these DT dogs and may contribute to a better understanding of the gastrointestinal disorders found in patients with DMD. Anat Rec, 301:673-685, 2018. (c) 2017 Wiley Periodicals, Inc. (AU)

FAPESP's process: 12/00259-1 - Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study
Grantee:Patricia Castelucci
Support type: Regular Research Grants
FAPESP's process: 08/05314-5 - Study of the chemical coding and P2X2 and P2X7 purina receptors of the ileum enteric nervous system of rats submitted to ischemia/reperfusion intestinaL of the ileal artery
Grantee:Patricia Castelucci
Support type: Regular Research Grants