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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Submucosal neurons and enteric glial cells expressing the P2X7 receptor in rat experimental colitis

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Author(s):
da Silva, Marcos Vinicius ; Marosti, Aline Rosa ; Mendes, Cristina Eusebio ; Palombit, Kelly ; Castelucci, Patricia
Total Authors: 5
Document type: Journal article
Source: ACTA HISTOCHEMICA; v. 119, n. 5, p. 481-494, 2017.
Web of Science Citations: 3
Abstract

The aim of this study was to evaluate the effect of ulcerative colitis on the submucosal neurons and glial cells of the submucosal ganglia of rats. 2,4,6-Trinitrobenzene sulfonic acid (TNBS; colitis group) was administered in the colon to induce ulcerative colitis, and distal colons were collected after 24 h. The colitis rats were compared with those in the sham and control groups. Double labelling of the P2X7 receptor with calbindin (marker for intrinsic primary afferent neurons, IPANs, submucosal plexus), calretinin (marker for secretory and vasodilator neurons of the submucosal plexus), HuC/D and S100 beta was performed in the submucosal plexus. The density (neurons per area) of submucosal neurons positive for the P2X7 receptor, calbindin, calretinin and HuC/D decreased by 21%, 34%, 8.2% and 28%, respectively, in the treated group. In addition, the density of enteric glial cells in the submucosal plexus decreased by 33%. The profile areas of calbindin-immunoreactive neurons decreased by 25%. Histological analysis revealed increased lamina propria and decreased collagen in the colitis group. This study demonstrated that ulcerative colitis affected secretory and vasodilatory neurons, IPANs and enteric glia of the submucosal plexus expressing the P2X7 receptor. (AU)

FAPESP's process: 12/00259-1 - Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study
Grantee:Patricia Castelucci
Support type: Regular Research Grants
FAPESP's process: 08/05314-5 - Study of the chemical coding and P2X2 and P2X7 purina receptors of the ileum enteric nervous system of rats submitted to ischemia/reperfusion intestinaL of the ileal artery
Grantee:Patricia Castelucci
Support type: Regular Research Grants