| Texto completo | |
| Autor(es): |
Rezende, Nayara
[1]
;
Jayme, Cristiano Ceron
[2, 1]
;
Brassesco, Maria Sol
[3]
;
Tedesco, Antonio Claudio
[2, 1]
;
de Oliveira, Harley Francisco
[4]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Croup, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Lab Cell Biol & Oncogenet, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med Clin Radiotherapy Serv, BR-14048900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Photodiagnosis and Photodynamic Therapy; v. 26, p. 371-374, JUN 2019. |
| Citações Web of Science: | 2 |
| Resumo | |
The widely accepted resazurin-based assay can be used, prior to in vivo studies, as an inexpensive method to determine cytotoxicity. The aim of this study was to evaluate and standardize the assay conditions for oral squamous cancer cell (OSCC) and glioblastoma (U87-MG) lines by UV-vis spectroscopy. The cells were treated with 25 mu gmL(-1) of resazurin sodium salt and then incubated for 4 h, 6 h, and 6.5 h. All absorbance measurements were carried out at 21 +/- 1 degrees C on a spectrophotometer with a microplate reader. After 4-hs of incubation, resazurin was completely reduced by OSCC cells, as demonstrated by the suppression of the absorbance at 380 nm. However, the U87-MG cells needed 6.5 h of incubation to demonstrate the same behavior. The Statistical analysis did not indicate significant differences between the OSCC and U87-MG cell lines' viability after 4 and 6.5 h respectively. We concluded that spectroscopic analysis is an efficient method for the standardization of the resazurin assay. In addition, without the implementation of suitable protocols, there could be an increase in the chance of errors or false positives or negatives that would reduce the usefulness of the data. (AU) | |
| Processo FAPESP: | 18/10237-1 - Desenvolvimento e caracterização de scaffolds à base de PCL-PGE e DNA aplicados à engenharia de tecidos para regeneração óssea |
| Beneficiário: | Cristiano Ceron Jayme |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central |
| Beneficiário: | Antonio Claudio Tedesco |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 14/09421-1 - Estudo dos quimioterápicos radiossensibilizadores cisplatina e temozolomida veiculados em sistemas de liberação de fármacos |
| Beneficiário: | Harley Francisco de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |