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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Standardization of a resazurin-based assay for the evaluation of metabolic activity in oral squamous carcinoma and glioblastoma cells

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Rezende, Nayara [1] ; Jayme, Cristiano Ceron [2, 1] ; Brassesco, Maria Sol [3] ; Tedesco, Antonio Claudio [2, 1] ; de Oliveira, Harley Francisco [4]
Total Authors: 5
[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photobiol & Photomed Res Croup, Dept Chem, Ctr Nanotechnol & Tissue Engn, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Lab Cell Biol & Oncogenet, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Med Clin Radiotherapy Serv, BR-14048900 Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 26, p. 371-374, JUN 2019.
Web of Science Citations: 2

The widely accepted resazurin-based assay can be used, prior to in vivo studies, as an inexpensive method to determine cytotoxicity. The aim of this study was to evaluate and standardize the assay conditions for oral squamous cancer cell (OSCC) and glioblastoma (U87-MG) lines by UV-vis spectroscopy. The cells were treated with 25 mu gmL(-1) of resazurin sodium salt and then incubated for 4 h, 6 h, and 6.5 h. All absorbance measurements were carried out at 21 +/- 1 degrees C on a spectrophotometer with a microplate reader. After 4-hs of incubation, resazurin was completely reduced by OSCC cells, as demonstrated by the suppression of the absorbance at 380 nm. However, the U87-MG cells needed 6.5 h of incubation to demonstrate the same behavior. The Statistical analysis did not indicate significant differences between the OSCC and U87-MG cell lines' viability after 4 and 6.5 h respectively. We concluded that spectroscopic analysis is an efficient method for the standardization of the resazurin assay. In addition, without the implementation of suitable protocols, there could be an increase in the chance of errors or false positives or negatives that would reduce the usefulness of the data. (AU)

FAPESP's process: 18/10237-1 - Development and characterization of scaffolds based on PCL - PGE and DNA applied to tissue engineering for bone regeneration
Grantee:Cristiano Ceron Jayme
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/50181-1 - Use of drugs containing nanocarriers with photosensitizers and/or other active compounds applied to cell therapy and treatment of central nervous system disorders
Grantee:Antonio Claudio Tedesco
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/09421-1 - Study of the radiosensitizers chemotherapeutic cisplatin and temozolomide carried in drug delivery systems
Grantee:Harley Francisco de Oliveira
Support type: Regular Research Grants