Determination of Ethofumesate-2-hydroxy Stereolability by Dynamic HPLC

Autor(es)

Perovani, Icaro Salgado ^[1] ; Moraes de Oliveira, Anderson Rodrigo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SEPARATION SCIENCE PLUS; v. 1, n. 12, p. 771-775, DEC 2018.
• Citações Web of Science: 0

Resumo

Evaluating stereolability of target chiral chemical is pivotal when developing enantioselective studies. Ethofumesate-2-hydroxy is one of the main metabolites of ethofumesate, a chiral herbicide currently marketed as racemate. In order to perform enantioselective pharmacokinetic and metabolism studies, the stereolability of susceptible molecules should be evaluated. Based on that, dynamic high-performance liquid chromatography experiments were performed to evaluate ethofumesate-2-hydroxy enantiomerization process on-column. The thermodynamic results helped understand that ethofumesate-2-hydroxy enantiomerization is a bimolecular process assisted by acid and base catalysis. The enantiomerization barrier determined suggested that ethofumesate-2-hydroxy single enantiomers may undergo extensive racemization at ambient temperature turning ethofumesate-2-hydroxy enantioselective studies into an unfeasible task. (AU)

• Processo FAPESP: 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/07534-4 - Desenvolvimento de métodos cromatográficos/eletroforéticos para posterior aplicação em estudos in vitro de inibição enzimática e predição de interações medicamentosas de praguicidas quirais - Fase 2
• Beneficiário: Anderson Rodrigo Moraes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular