Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth

Autor(es)

Giovani, Priscila A. ^[1] ; Salmon, Cristiane R. ^[2] ; Martins, Lucian ^[2] ; Leme, Adriana F. P. ^[3] ; Puppin-Rontani, Regina M. ^[1] ; Mofatto, Luciana S. ^[4] ; Nociti Jr, Francisco H. ; Kantovitz, Kamila R. ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodont, Div Periodont, Piracicaba, SP - Brazil

^[3] CNPEM, LNBio, Brazilian Biosci Natl Lab, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Piracicaba, SP - Brazil

^[5] Sao Leopoldo Mand Res Ctr, Dept Dent Mat, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 90, n. 7, p. 775-787, JUL 2019.
• Citações Web of Science: 0

Resumo

Background Physiological roles for the periodontal ligament (PDL) include tooth eruption and anchorage, force absorption, and provision of proprioceptive information. Despite the advances in understanding the biology of PDL cells, there is a lack of information regarding the molecular signature of deciduous (DecPDL) and permanent (PermPDL) PDL tissues. Thus, the present study was designed to characterize the membrane proteome of DecPDL and PermPDL cells. Methods Primary PDL cells were obtained (n = 6) and a label-free quantitative proteome of cell membrane-enriched components was performed. Proteome findings were validated by quantitative polymerase chain reaction and Western blot assays in fresh human tissues (n = 8) and primary cell cultures (n = 6). In addition, confocal microscopy was used to verify the expression of target factors in the PDL cell cultures. Results Comparative gene ontology enrichment analysis evidenced that most stickling differences involved ``endomembrane system{''} (PICALM, STX4, and LRP10), ``hydrolase activity{''} (NCSTN and XRCC6), ``protein binding{''} (PICALM, STX4, GPNMB, VASP, extended-synaptotagmin 2 {[}ESYT2], and leucine-rich repeat containing 15 {[}LRRC15]), and ``isomerase activity{''} (FKBP8). Data are available via ProteomeXchange with identifier PXD010226. At the transcript level, high PICALM in DecPDL and ESYT2 and LRRC15 in PermPDL were confirmed in fresh PDL tissues. Furthermore, Western blot analysis confirmed increased levels of PICALM, LRRC15, and ESYT2 in cells and/or fresh tissues, and confocal microscopy confirmed the trends for PICALM and LRRC15 expression in PDL cells. Conclusion We report the first comprehensive characterization of the membrane protein machinery of DecPDL and PermPDL cells, and together, we identified a distinct molecular signature for these cell populations, including unique proteins for DecPDL and PermPDL. (AU)

• Processo FAPESP: 16/13786-0 - Influência de Nova Nanotecnologia nas Propriedades Físico-Químicas-Biológicas do Cimento de Ionômero de Vidro Convencional
• Beneficiário: Kamila Rosamilia Kantovitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/02942-1 - Análise Global do Protema do Ligamento Periodontal.
• Beneficiário: Priscila Alves Giovani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/06372-2 - Determinação do papel dos cementócitos na homeostasia do cemento dental
• Beneficiário: Francisco Humberto Nociti Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular