Label-Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Autor(es)

Salvato, Fernando ^[1] ; Loziuk, Philip ^[2] ; Kiyota, Eduardo ^[1] ; Daneluzzi, Gabriel Silva ^[3] ; Araujo, Pedro ^[1] ; Muddiman, David C. ^[2] ; Mazzafera, Paulo ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13081 Campinas, SP - Brazil

^[2] North Carolina State Univ, Dept Chem, WM Keck FTMS Lab, Raleigh, NC 27695 - USA

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROTEOMICS; v. 19, n. 14 JUL 2019.
• Citações Web of Science: 1

Resumo

Drought is considered the major abiotic stress limiting crop productivity. This study seeks to identify proteins involved in the drought response in sugarcane stems submitted to drought stress. The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified. A total of 1204 differentially accumulated proteins are detected in response to drought, among which 586 and 618 are increased and reduced in abundance, respectively. A total of 115 exclusive proteins are detected, being 41 exclusives of drought-stressed plants and 74 exclusives of control plants. In the control plants, most of these proteins are related to cell wall metabolism, indicating that drought affects negatively the cell wall metabolism. Also, 37 transcription factors (TFs) are identified, which are low abundant nuclear proteins and are differentially accumulated in response to drought stress. These TFs are associated to protein domains such as leucine-rich (bZIP), C2H2, NAC, C3H, LIM, Myb-related, heat shock factor (HSF) and auxin response factor (ARF). Increased abundance of chromatin remodeling and RNA processing proteins are also observed. It is suggested that these variations result from an imbalance of protein synthesis and degradation processes induced by drought. (AU)

• Processo FAPESP: 16/06236-4 - Identificação e quantificação absoluta de fatores de transcrição envolvidos na biossíntese de lignina em colmos de cana-de-açúcar submetidos a estresse hídrico
• Beneficiário: Fernanda Salvato
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 13/10779-5 - Proteoma do colmo e do núcleo de células de cana-de-açúcar (Saccharum spp.) associado ao controle da biossíntese de lignina
• Beneficiário: Fernanda Salvato
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/25994-1 - Lignina em espécies de Saccharum spp.
• Beneficiário: Juan Pablo Portilla Llerena
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Beneficiário: Paulo Mazzafera
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático