Label-Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress

Author(s)

Salvato, Fernando ^[1] ; Loziuk, Philip ^[2] ; Kiyota, Eduardo ^[1] ; Daneluzzi, Gabriel Silva ^[3] ; Araujo, Pedro ^[1] ; Muddiman, David C. ^[2] ; Mazzafera, Paulo ^{[1, 3]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13081 Campinas, SP - Brazil

^[2] North Carolina State Univ, Dept Chem, WM Keck FTMS Lab, Raleigh, NC 27695 - USA

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: PROTEOMICS; v. 19, n. 14 JUL 2019.
• Web of Science Citations: 1

Abstract

Drought is considered the major abiotic stress limiting crop productivity. This study seeks to identify proteins involved in the drought response in sugarcane stems submitted to drought stress. The integration of nuclei enrichment sample preparation with the shotgun proteomic approach results in great coverage of the sugarcane stem proteome with 5381 protein groups identified. A total of 1204 differentially accumulated proteins are detected in response to drought, among which 586 and 618 are increased and reduced in abundance, respectively. A total of 115 exclusive proteins are detected, being 41 exclusives of drought-stressed plants and 74 exclusives of control plants. In the control plants, most of these proteins are related to cell wall metabolism, indicating that drought affects negatively the cell wall metabolism. Also, 37 transcription factors (TFs) are identified, which are low abundant nuclear proteins and are differentially accumulated in response to drought stress. These TFs are associated to protein domains such as leucine-rich (bZIP), C2H2, NAC, C3H, LIM, Myb-related, heat shock factor (HSF) and auxin response factor (ARF). Increased abundance of chromatin remodeling and RNA processing proteins are also observed. It is suggested that these variations result from an imbalance of protein synthesis and degradation processes induced by drought. (AU)

• FAPESP's process: 16/06236-4 - Identification and absolute quantification of transcription factors involved in lignin biosynthesis in sugarcane stalks under drought stress
• Grantee: Fernanda Salvato
• Support type: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/25994-1 - Lignin in species of the genus Saccharum
• Grantee: Juan Pablo Portilla Llerena
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Grantee: Paulo Mazzafera
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 13/10779-5 - Stalk and nuclear proteomes of sugarcane (Saccharum spp.) associated to the control of lignin biosynthesis
• Grantee: Fernanda Salvato
• Support type: Scholarships in Brazil - Post-Doctorate