Bone growth as the main determinant of mouse digit tip regeneration after amputation

Autor(es)

Sensiate, L. A. ^[1] ; Marques-Souza, H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biochem & Tissue Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUL 4 2019.
• Citações Web of Science: 1

Resumo

Regeneration is classically demonstrated in mammals using mice digit tip. In this study, we compared different amputation plans and show that distally amputated digits regrow with morphology close to normal but fail to regrow the fat pad. Proximally amputated digits do not regrow the phalangeal bone, but the remaining structures (nail, skin and connective tissue), all with intrinsic regenerative capacity, re-establishing integrity indistinguishably in distally and proximally amputated digits. Thus, we suggest that the bone growth promoted by signals and progenitor cells not removed by distal amputations is responsible for the re-establishment of a drastically different final morphology after distal or proximal digit tip amputations. Despite challenging the use of mouse digit tip as a model system for limb regeneration in mammals, these findings evidence a main role of bone growth in digit tip regeneration and suggest that mechanisms that promote joint structures formation should be the main goal of regenerative medicine for limb and digit regrowth. (AU)

• Processo FAPESP: 19/09870-4 - Crescimento ósseo como o principal determinante da regeneração após amputação da extremidade do dígito de camundongos
• Beneficiário: Henrique Marques Barbosa de Souza
• Linha de fomento: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 12/09602-0 - Caracterização da rede de regulação cardiogênica controlada por Coup-TFII a partir de células-tronco embrionárias
• Beneficiário: Henrique Marques Barbosa de Souza
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores