Validation of an analytical method for the determination of the main ayahuasca active compounds and application to real ayahuasca samples from Brazil

Autor(es)

Souza, Rita C. Z. ^[1] ; Zandonadi, Flavia S. ^[1] ; Freitas, Donizete P. ^[1] ; Tofoli, Luis F. F. ^[2] ; Sussulini, Alessandra ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Chem, Lab Bioanalyt & Integrated Omics LaBIOmics, POB 6154, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Fac Med Sci, Dept Psychiat, BR-13025251 Campinas, SP - Brazil

^[3] Univ Campinas UNICAMP, Inst Chem, Natl Inst Sci & Technol Bioanalyt INCTBio, POB 6154, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1124, p. 197-203, AUG 15 2019.
• Citações Web of Science: 0

Resumo

Ayahuasca is a brew prepared from the water decoction of two Amazonian plants, which is legally used for religious, cultural or therapeutic activities. The potential use of ayahuasca as a natural or phytotherapeutic drug is directly linked to the action of its active compounds and their connection with the therapeutic efficacy of the beverage. In this context, the aim of the present study was to establish a selective, sensitive and reproducible analytical method for the quantification of the main active ayahuasca compounds. Thirty-eight samples from the state of Sao Paulo, Brazil, were analyzed and the simultaneous quantifications of N,N-dimethyltryptamine (DMT), tetrahydroharmine (THH), harmine (HME) and harmaline (HML) were performed. This study enabled the development of a fast validated analytical method with minimal matrix interference and high reproducibility for the tracing of active ayahuasca compound concentrations for the first time. This method is important as an auxiliary tool for the study of active compound effects in biological responses using different multi-omic platforms. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/01525-3 - Estudos metabolômicos de pacientes com depressão tratados com ayahuasca
• Beneficiário: Alessandra Sussulini
• Modalidade de apoio: Auxílio à Pesquisa - Regular