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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

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Autor(es):
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Guilherme, Viviane A. [1] ; Ribeiro, Ligia N. M. [1] ; Alcantara, Ana C. S. [2] ; Castro, Simone R. [1] ; Rodrigues da Silva, Gustavo H. [1] ; da Silva, Camila Goncalves [1] ; Breitkreitz, Marcia C. [3] ; Clemente-Napimoga, Juliana [4, 5] ; Macedo, Cristina G. [4] ; Abdalla, Henrique B. [4] ; Bonfante, Ricardo [4] ; Cereda, Cintia M. S. [1, 5] ; de Paula, Eneida [1]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[2] Fed Univ Maranhao UFMA, Dept Chem, Sao Luis, MA - Brazil
[3] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[5] Inst Pesquisas Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Campinas, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
Citações Web of Science: 0
Resumo

Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (2(3)) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 degrees C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1 beta and TNF-alpha), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints. (AU)

Processo FAPESP: 13/10766-0 - Naproxeno encapsulado em carreadores lipídicos nanoestruturados para administração parenteral: do preparo aos testes farmacológicos
Beneficiário:Viviane Aparecida Guilherme Damasio
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
Beneficiário:Eneida de Paula
Linha de fomento: Auxílio à Pesquisa - Temático