Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Full text
Author(s):
Show less -
Guilherme, Viviane A. [1] ; Ribeiro, Ligia N. M. [1] ; Alcantara, Ana C. S. [2] ; Castro, Simone R. [1] ; Rodrigues da Silva, Gustavo H. [1] ; da Silva, Camila Goncalves [1] ; Breitkreitz, Marcia C. [3] ; Clemente-Napimoga, Juliana [4, 5] ; Macedo, Cristina G. [4] ; Abdalla, Henrique B. [4] ; Bonfante, Ricardo [4] ; Cereda, Cintia M. S. [1, 5] ; de Paula, Eneida [1]
Total Authors: 13
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
[2] Fed Univ Maranhao UFMA, Dept Chem, Sao Luis, MA - Brazil
[3] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, Campinas, SP - Brazil
[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil
[5] Inst Pesquisas Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
Web of Science Citations: 0
Abstract

Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (2(3)) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 degrees C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1 beta and TNF-alpha), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints. (AU)

FAPESP's process: 13/10766-0 - Naproxen encapsulated in nanostructured lipid carriers for parenteral administration: preparation, characterization and pharmacological evaluation.
Grantee:Viviane Aparecida Guilherme Damasio
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics
Grantee:Eneida de Paula
Support Opportunities: Research Projects - Thematic Grants