Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration

Autor(es):Mostrar menos -

Guilherme, Viviane A. ^[1] ; Ribeiro, Ligia N. M. ^[1] ; Alcantara, Ana C. S. ^[2] ; Castro, Simone R. ^[1] ; Rodrigues da Silva, Gustavo H. ^[1] ; da Silva, Camila Goncalves ^[1] ; Breitkreitz, Marcia C. ^[3] ; Clemente-Napimoga, Juliana ^{[4, 5]} ; Macedo, Cristina G. ^[4] ; Abdalla, Henrique B. ^[4] ; Bonfante, Ricardo ^[4] ; Cereda, Cintia M. S. ^{[1, 5]} ; de Paula, Eneida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Fed Univ Maranhao UFMA, Dept Chem, Sao Luis, MA - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[5] Inst Pesquisas Sao Leopoldo Mandic, Fac Sao Leopoldo Mandic, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, AUG 1 2019.
• Citações Web of Science: 0

Resumo

Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (2(3)) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 degrees C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1 beta and TNF-alpha), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints. (AU)

• Processo FAPESP: 13/10766-0 - Naproxeno encapsulado em carreadores lipídicos nanoestruturados para administração parenteral: do preparo aos testes farmacológicos.
• Beneficiário: Viviane Aparecida Guilherme Damasio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
• Beneficiário: Eneida de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Temático