Effects of subcutaneous injection of ozone during wound healing in rats

Autor(es):Mostrar menos -

Soares, Ciro D. ^[1] ; Morais, Thayna M. L. ^[1] ; Araujo, Roberta M. F. G. ^[2] ; Meyer, Patricia F. ^[3] ; Oliveira, Eric A. F. ^[3] ; Silva, V, Rodrigo M. ; Carreiro, Eneida M. ^[4] ; Carreiro, Edvaldo P. ^[4] ; Belloco, Veronica G. ^[5] ; Mariz, Bruno A. L. A. ^[1] ; Jorge-Junior, Jacks ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Oral Pathol Sect, Dept Oral Diag, Av Limeira 901, POB 52, BR-1314903 Piracicaba, SP - Brazil

^[2] Univ Potiguar, Med Dept, Natal, RN - Brazil

^[3] Univ Potiguar, Physiotherapy Dept, Natal, RN - Brazil

^[4] Univ Ctr Rio Grande do Norte, Physiotherapy Dept, Natal, RN - Brazil

^[5] FisioKine Ctr, Physiotherapy Dept, Buenos Aires, DF - Argentina

• Tipo de documento: Artigo Científico
• Fonte: GROWTH FACTORS; v. 37, n. 1-2 JULY 2019.
• Citações Web of Science: 0

Resumo

Fibroblast growth factor 2 (FGF2) regulates the wound repair process and it is secreted by inflammatory and endothelial cells, and by myofibroblasts. This study aimed to establish the expression patterns of FGF2 and myofibroblastic differentiation during wound healing in rats treated with subcutaneous ozone injection. We created full-thickness excisional wounds in rats, and the healing process was analyzed through morphometric analyses and digital quantification of immunoreactivity of smooth muscle actin and FGF2. Ozone therapy-treated wounds presented granulation tissue with a reduced number of inflammatory cells and greater dermal cellularity, and intense collagen deposition. FGF2 immunoreactivity, microvessel density, and amount of myofibroblasts were significantly higher in treated wounds compared to controls. In conclusion, it was demonstrated that subcutaneous injections of ozone accelerate and ameliorate wound repairing process. Moreover, injectable ozone therapy's action mechanism may be associated with FGF2 overexpression. (AU)

• Processo FAPESP: 17/16102-8 - Papel biológico de RUNX1, Akt, COX-2 e metaloproteinases no mecanismo de metástase em melanomas
• Beneficiário: Jacks Jorge Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/25905-1 - Expressão de Akt, COX-2, RUNX1 e metaloproteinases em melanomas metastáticos e Prospecção biotecnológica de moléculas com atividade antitumoral
• Beneficiário: Ciro Dantas Soares
• Modalidade de apoio: Bolsas no Brasil - Doutorado