Assessing the cytotoxic potential of glycoalkaloidic extract in nanoparticles against bladder cancer cells

Autor(es):Mostrar menos -

Miranda, Mariza A. ^[1] ; Marcato, Priscyla D. ^[1] ; Carvalho, Ivana P. S. ^[1] ; Silva, Leticia B. ^[1] ; Ribeiro, Diego L. ^[2] ; Amaral, Robson ^[1] ; Swiech, Kamilla ^[1] ; Bastos, Jairo K. ^[1] ; Paschoal, Jonas A. R. ^[1] ; dos Reis, Rodolfo B. ^[3] ; Bentley, Maria V. L. B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Surg & Anat, Div Urol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Pharmacy and Pharmacology; v. 71, n. 10 AUG 2019.
• Citações Web of Science: 0

Resumo

Objective This study proposed to use the nanotechnology to deliver glycoalkaloidic extract (AE) to bladder cancer cells, evaluating their activity in 2D and 3D models and the biological mechanism of cell death. Methods NPs were prepared by nanoprecipitation method using polylactic acid (PLA) and characterized considering their size, charge, particle concentration and stability. The cytotoxicity was evaluated in 2D and 3D model, and the apoptosis and cell cycle were investigated using flow cytometry. Key findings NPs loading AE (NP-AE) had diameter around 125 +/- 6 nm (PdI <0.1) and negative charge. The encapsulation efficiency of SM and SS was higher than 85% for both compounds. The obtained formulation showed a significant in-vitro cytotoxic effect against RT4 cells in a dose-dependent manner with IC50 two fold lower than the free AE. The cytotoxic effect of NP-AE was mediated by apoptosis and cell cycle arrested in the S phase. RT4 cells cultured under 3D conditions exhibited a higher resistance to the treatments (IC50 similar to three fold higher than in 2D cell culture). Conclusion The NP-AE might be a promising nanocarrier to load and deliver glycoalkaloids against bladder cancer. (AU)

• Processo FAPESP: 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar
• Beneficiário: Maria Vitória Lopes Badra Bentley
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/14665-0 - Desenvolvimento de nanopartículas funcionalizadas com folato para o carreamento de glicoalcalóides de Solanum lycocarpum e avaliação biológica em modelos de cultivo celular 2D e 3D
• Beneficiário: Mariza Abreu Miranda
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado