Candida albicans-Cell Interactions Activate Innate Immune Defense in Human Palate Epithelial Primary Cells via Nitric Oxide (NO) and (3-Defensin 2 (hBD-2)

Autor(es):Mostrar menos -

Casaroto, Ana Regina ^[1] ; da Silva, Rafaela Alves ^[1] ; Salmeron, Samira ^[2] ; Rubo de Rezende, Maria Lucia ^[2] ; Dionisio, Thiago Jose ^[3] ; dos Santos, Carlos Ferreira ^[3] ; Pinke, Karen Henriette ^[1] ; Guarizo Klingbeil, Maria Fatima ^[4] ; Salomao, Priscila Aranda ^[1] ; Ribeiro Lopes, Marcelo Milanda ^[1] ; Lara, Vanessa Soares ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Surg Stomatol Pathol & Radiol, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodontol, BR-17012901 Bauru, SP - Brazil

^[3] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[4] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-03178200 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 8, n. 7 JUL 2019.
• Citações Web of Science: 0

Resumo

The presence of Candida albicans in the biofilm underlying the dental prosthesis is related to denture stomatitis (DS), an inflammatory reaction of the oral mucosa. The oral epithelium, a component of the innate immune response, has the ability to react to fungal invasion. In this study, we evaluated the in vitro effect of viable C. albicans on the apoptosis, nitric oxide (NO) production, and beta-defensin 2 (hBD-2) expression and production of human palate epithelial cells (HPECs). We further determined whether or not these effects were correlated with fungal invasion of epithelial cells. Interaction between HPEC primary culture and C. albicans was obtained through either direct or indirect cell cell contact with a supernatant from a hyphal fungus. We found that the hyphae supernatants were sufficient to induce slight HPEC apoptosis, which occurred prior to the activation of the specific mechanisms of epithelial defense. The epithelial defense responses were found to occur via NO and antimicrobial peptide hBD-2 production only during direct contact between C. albicans and HPECs and coincided with the fungus's intraepithelial invasion. However, although the hBD-2 levels remained constant in the HPEC supernatants over time, the NO release and hBD-2 gene expression were reduced at a later time (10 h), indicating that the epithelial defense capacity against the fungal invasion was not maintained in later phases. This aspect of the immune response was associated with increased epithelial invasion and apoptosis maintenance. (AU)

• Processo FAPESP: 11/17481-6 - Estudo in vitro da produção de peptídeos antimicrobianos por células epiteliais de palato humano em resposta a Candida albicans
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03965-2 - Papel do sistema renina-angiotensina em diferentes modelos inflamatórios orais: uma abordagem interdisciplinar experimental e clínica
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Temático