Simultaneous zika and dengue serotype-4 viral detection and isolation from a donor plasma unit

Autor(es)

Slavov, Svetoslav Nanev ^{[1, 2]} ; Ferreira, Fernando Ursoli ^[2] ; Rodrigues, Evandra Strazza ^[2] ; Gomes, Rogerio ^[2] ; Covas, Dimas Tadeu ^{[1, 2]} ; Kashima, Simone ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Internal Med, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Reg Blood Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF VECTOR BORNE DISEASES; v. 56, n. 2, p. 166-169, APR-JUN 2019.
• Citações Web of Science: 0

Resumo

During zika and dengue viruse (ZIKV and DENV) outbreaks. the majority of the infected individuals remain clinically asymptomatic. Such asymptomatic individuals may occasionally acquire both arboviruses, donate blood, and contaminate haemoderivatives. The aim of this study was to characterize a ZIKViDENV-4 coinfection in asymptomatic blood donor who donated blood during a large mixed ZIKV/DENV outbreak in the Sao Paulo State, Brazil. On the basis of post-donation information, one blood donor sample was found positive for ZIKV and DENV RNA. The DENV molecular serotyping was performed by molecular testing. The sample was also titrated on VERO E6 cells in order to define the presence of infectious arboviruses. The real-time PCR testing of the blood donor sample demonstrated very high viral load for both ZIKV and DENV. Further, molecular serotyping of DENV showed that the presence of DENV-4. The viral titration in cell culture indicated a titre of 2.75 x10(6) PFU/ml which was concordant with the presence of infectious viruses in the blood donation. This is an interesting report for the simultaneous presence of infectious ZIKV and DENV-4 in asymptomatic blood sample. Special attention must be paid during mixed arboviral outbreaks for the possibility of transfusion-transmission of multiple arboviral agents. (AU)

• Processo FAPESP: 09/16623-1 - Mecanismos moleculares e consequências clínicas da infecção causada pelo Parvovírus B19 e Citomegalovírus humanos em pacientes com hemoglobinopatias e hemofilia
• Beneficiário: Svetoslav Nanev Slavov
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado