Increased whitening efficacy and reduced cytotoxicity are achieved by the chemical activation of a highly concentrated hydrogen peroxide bleaching gel

Autor(es)

Soares, Diana Gabriela ^[1] ; Marcomini, Natalia ^[2] ; de Oliveira Duque, Carla Caroline ^[3] ; Bordini, EsterAlves Ferreira ^[3] ; Zuta, Uxua Ortecho ^[3] ; Basso, Fernanda Goncalves ^[4] ; Hebling, Josimeri ^[5] ; de Souza Costa, Carlos Alberto ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Bauru, Dept Dentist Endodontia & Mat Odontol, Bauru, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Dentist Restauradora, Araraquara, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Mat Dent & Protese, Araraquara, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Fisiol & Patol, Araraquara, SP - Brazil

^[5] Univ Estadual Paulista, UNESP, Fac Odontol Araraquara, Dept Clin Infantil, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 27, 2019.
• Citações Web of Science: 1

Resumo

Abstract Objective This study was designed for the chemical activation of a 35% hydrogen peroxide (H2O2) bleaching gel to increase its whitening effectiveness and reduce its toxicity. Methodology First, the bleaching gel - associated or not with ferrous sulfate (FS), manganese chloride (MC), peroxidase (PR), or catalase (CT) - was applied (3x 15 min) to enamel/dentin discs adapted to artificial pulp chambers. Then, odontoblast-like MDPC-23 cells were exposed for 1 h to the extracts (culture medium + components released from the product), for the assessment of viability (MTT assay) and oxidative stress (H2DCFDA). Residual H2O2 and bleaching effectiveness (DE) were also evaluated. Data were analyzed with one-way ANOVA complemented with Tukey’s test (n=8. p<0.05). Results All chemically activated groups minimized MDPC-23 oxidative stress generation; however, significantly higher cell viability was detected for MC, PR, and CT than for plain 35% H2O2 gel. Nevertheless, FS, MC, PR, and CT reduced the amount of residual H2O2 and increased bleaching effectiveness. Conclusion Chemical activation of 35% H2O2 gel with MC, PR, and CT minimized residual H2O2 and pulp cell toxicity; but PR duplicated the whitening potential of the bleaching gel after a single 45-minute session. (AU)

• Processo FAPESP: 15/21770-4 - Desenvolvimento de produtos clareadores dentais experimentais baseados em princípios dos processos oxidativos avançados: análise dos efeitos estéticos e biológicos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular