| Texto completo | |
| Autor(es): Mostrar menos - |
Castro Olimpio, Regiane Marques
[1]
;
Fontes Moretto, Fernanda Cristina
[1]
;
De Sibio, Maria Teresa
[1]
;
de Oliveira, Miriane
[1]
;
Mathias, Lucas Solla
[1]
;
Goncalves, Bianca Mariani
[1]
;
Depra, Igor Carvalho
[1]
;
Tilli, Helena Paim.
[1]
;
Rodrigues, Bruna Moretto
[1]
;
Saraiva, Patricia Pinto
[1]
;
Maria, Durvanei Augusto
[2]
;
Nogueira, Celia Regina
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618970 Botucatu, SP - Brazil
[2] Butantan Inst, Biochem & Biophys Lab, 1500 Ave Vital Brazil, Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Life Sciences; v. 231, AUG 15 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
Triiodothyronine (T-3) and estrogen (E-2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa beta (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E-2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E-2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T-3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E-2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E-2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T-3. E-2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T-3. The protective effect of E-2 on the RANKL and OPG signaling pathway was demonstrated. E-2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E-2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T-3 is in excess. (AU) | |
| Processo FAPESP: | 14/16406-9 - AVALIAÇÃO DA AÇÃO DO ESTRÓGENO E DO HORMÔNIO TIREOIDIANO NA EXPRESSÃO DOS RNAs NÃO CODIFICANTES EM CÉLULAS OSTEOBLÁSTICAS DERIVADAS DO TECIDO ADIPOSO |
| Beneficiário: | Celia Regina Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |