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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts

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Author(s):
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Castro Olimpio, Regiane Marques [1] ; Fontes Moretto, Fernanda Cristina [1] ; De Sibio, Maria Teresa [1] ; de Oliveira, Miriane [1] ; Mathias, Lucas Solla [1] ; Goncalves, Bianca Mariani [1] ; Depra, Igor Carvalho [1] ; Tilli, Helena Paim. [1] ; Rodrigues, Bruna Moretto [1] ; Saraiva, Patricia Pinto [1] ; Maria, Durvanei Augusto [2] ; Nogueira, Celia Regina [1]
Total Authors: 12
Affiliation:
[1] Univ Estadual Paulista UNESP, Botucatu Med Sch, Dept Internal Med, BR-18618970 Botucatu, SP - Brazil
[2] Butantan Inst, Biochem & Biophys Lab, 1500 Ave Vital Brazil, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Life Sciences; v. 231, AUG 15 2019.
Web of Science Citations: 0
Abstract

Triiodothyronine (T-3) and estrogen (E-2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa beta (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E-2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E-2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T-3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E-2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E-2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T-3. E-2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T-3. The protective effect of E-2 on the RANKL and OPG signaling pathway was demonstrated. E-2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E-2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T-3 is in excess. (AU)

FAPESP's process: 14/16406-9 - EVALUATION OF ACTION ESTROGEN AND THYROID HORMONE ACTION UPON NONCODING RNA EXPRESSION IN OSTEOBLASTIC CELLS DERIVED OF ADIPOSE TISSUE
Grantee:Celia Regina Nogueira
Support type: Regular Research Grants